Identification of miRNAs and Their Target Genes Associated with Sweet Corn Seed Vigor by Combined Small RNA and Degradome Sequencing
Identification of miRNAs and Their Target Genes Associated with Sweet Corn Seed Vigor by Combined Small RNA and Degradome Sequencing
复制标题
通过小 RNA 和降解组测序相结合鉴定与甜玉米种子活力相关的 miRNA 及其靶基因。
DOI：
10.1021/acs.jafc.5b00522
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发表时间：
2015-06-10
影响因子：
6.1
通讯作者：
Zhu, Cheng
中科院分区：
文献类型：
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作者：
Gong, Shumin;Ding, Yanfei;Zhu, Cheng
高活力种子对农业生产具有重要意义。甜玉米种子活力低，阻碍了甜玉米的推广。saccharata Sturt）。为了更好地理解miRNAs与种子活力的关系及其调控机制，通过小RNA测序技术构建了非人工老化和人工老化2天的种子小RNA文库。共发现27个差异表达的miRNAs，其中10个通过实时定量聚合酶链反应进一步证实。此外，通过degradome测序鉴定miRNA的靶标。共鉴定了1142个可能被131个miRNA切割的靶标。基因本体论（GO）注释结果表明，9种差异表达的miRNAs切割的26个靶基因可能在种子活力的调控中发挥作用，如PC-5 p-213179_17靶向的过氧化物酶超家族蛋白在氧化还原过程和氧化胁迫应答中发挥作用。这些发现为理解miRNAs与种子活力的关系提供了有价值的信息。
High seed vigor is significant for agriculture. Low seed vigor of sweet corn hindered the popularization of sweet corn (Zea mays L. saccharata Sturt). To better understand the involvement and regulatory mechanism of miRNAs with seed vigor, small RNA libraries from seeds non-artificially aged and artificially aged for 2 days were generated by small RNA sequencing. A total of 27 differentially expressed miRNAs were discovered, of which 10 were further confirmed by real-time quantitative polymerase chain reaction. Furthermore, targets of miRNAs were identified by degradome sequencing. A total of 1142 targets that were potentially cleaved by 131 miRNAs were identified. Gene ontology (GO) annotations of target transcripts indicated that 26 target genes cleaved by 9 differentially expressed miRNAs might play roles in the regulation of seed vigor, such as peroxidase superfamily protein targeted by PC-5p-213179_17 playing a role in the oxidation-reduction process and response to oxidative stress. These findings provide valuable information to understand the involvement of miRNAs with seed vigor.