Serological detection of 2019-nCoV respond to the epidemic: A useful complement to nucleic acid testing
Serological detection of 2019-nCoV respond to the epidemic: A useful complement to nucleic acid testing
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DOI：
10.1016/j.intimp.2020.106861
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发表时间：
2020-11-01
影响因子：
5.6
通讯作者：
Zheng, Rui
中科院分区：
文献类型：
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作者：
Zhang, Jin;Zhang, Xiaoming;Zheng, Rui
冠状病毒病2019(新冠肺炎)已迅速传播到215多个国家，报告病例超过119万例，死亡超过54万人。快速诊断仍然是控制疫情的瓶颈。采用全自动化学发光免疫分析法检测742例受试者血清中2019年新冠状病毒抗体，以观察不同人群新冠肺炎抗体产生的动态过程及血清流行病学。新冠肺炎患者在出现症状后3-15天内出现特异性抗体反应(阳性)。随着疾病的发展，特异性IgM和IgG水平也随之升高。Ig M和Ig G的受试者工作特征曲线下面积分别为0.984和1.000。该抗体检测方法具有良好的敏感性和特异性。了解新冠肺炎患者的血清学动态变化和人群的血清流行病学情况，有助于进一步控制新冠肺炎疫情。
Corona Virus Disease 2019 (COVID-19) has spread rapidly to more than 215 countries, with over 11.91 million reported cases and more than 540,000 deaths. Rapid diagnosis remains a bottleneck for containing the epidemic. We used an automated chemiluminescent immunoassay to detect serum IgM and IgG antibodies to the 2019-nCoV in 742 subjects, so as to observe the dynamic process of antibody production in COVID-19 disease and seroepidemiology in different populations. Patients with COVID-19 were reactive (positive) for specific antibodies within 3-15 days after onset of symptoms. Specific IgM and IgG levels increased with the progression of the disease. The areas under the receiver operating characteristic curves for IgM and IgG were 0.984 and 1.000, respectively. This antibody detection assay had good sensitivity and specificity. The understanding of the dynamic serological changes of COVID-19 patients and the seroepidemiological situation of the population will be helpful to further control the epidemic of COVID-19.