Heat stress induces oxidative stress and activates the KEAP1-NFE2L2-ARE pathway in bovine endometrial epithelial cells
Heat stress induces oxidative stress and activates the KEAP1-NFE2L2-ARE pathway in bovine endometrial epithelial cells
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热应激诱导牛子宫内膜上皮细胞氧化应激，激活KEAP1-NFE2L2-ARE通路
DOI：
10.1093/biolre/ioab143
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发表时间：
2021-07-23
影响因子：
3.6
通讯作者：
Takahashi, Masashi
中科院分区：
文献类型：
--
作者：
Murata, Hirona;Kunii, Hiroki;Takahashi, Masashi
热应激会对奶牛的生殖功能产生不利影响。虽然热应激和氧化应激之间的关系已被提出，它还没有得到充分的验证，在牛子宫内膜上皮细胞。在这里，我们调查是否氧化应激是由热应激引起的牛子宫内膜上皮细胞在高温下。荧光素酶报告基因检测结果显示，高温培养的子宫内膜上皮细胞中热休克元件和抗氧化反应元件的报告基因活性比基础温度培养的细胞中的报告基因活性高。此外，核因子，红细胞2样2（NFE 2L2），细胞环境应激反应的主调节器，稳定和抗氧化酶基因的表达水平在高温下增加。免疫组化证实NFE 2L2在高温培养的子宫内膜上皮细胞中的核定位。定量聚合酶链反应分析显示，与基础温度培养的细胞相比，高温培养的子宫内膜上皮细胞中前列腺素内过氧化物合酶2（PTGS 2）和白细胞介素8等代表性炎症细胞因子基因的表达水平显着降低。因此，我们的研究结果表明，热应激诱导氧化应激，而NFE 2L2在热应激条件下培养的牛子宫内膜上皮细胞中起着保护作用。摘要热应激诱导氧化应激，而NFE 2L2，细胞环境应激反应的主调节剂，在热应激条件下培养的牛子宫内膜上皮细胞中具有保护作用。
Heat stress adversely affects the reproductive function in cows. Although a relationship between heat stress and oxidative stress has been suggested, it has not been sufficiently verified in bovine endometrial epithelial cells. Here, we investigated whether oxidative stress is induced by heat stress in bovine endometrial epithelial cells under high temperature. Luciferase reporter assays showed that the reporter activity of heat shock element and antioxidant responsive element was increased in endometrial epithelial cells cultured under high temperature compared to that in cells cultured under basal (thermoneutral) temperature. Also, nuclear factor, erythroid 2 like 2 (NFE2L2), a master regulator of cellular environmental stress response, stabilized and the expression levels of antioxidant enzyme genes increased under high temperature. Immunostaining confirmed the nuclear localization of NFE2L2 in endometrial epithelial cells cultured under high temperature. Quantitative polymerase chain reaction analysis showed that the expression levels of representative inflammatory cytokine genes, such as prostaglandin-endoperoxide synthase 2 (PTGS2) and interleukin 8, were significantly decreased in endometrial epithelial cells cultured under high temperature compared to those in cells cultured under basal temperature. Thus, our results suggest that heat stress induces oxidative stress, whereas NFE2L2 plays a protective role in bovine endometrial epithelial cells cultured under heat stress conditions.Summary sentenceHeat stress induces oxidative stress, whereas NFE2L2, a master regulator of the cellular environmental stress response, has a protective role in bovine endometrial epithelial cells cultured under heat stress conditions.