αβ lineage-specific expression of the α T cell receptor gene by nearby silencers
αβ lineage-specific expression of the α T cell receptor gene by nearby silencers
复制标题
附近沉默子对 α T 细胞受体基因的 αβ 谱系特异性表达
DOI：
10.1016/0092-8674(89)90010-x
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发表时间：
1989
期刊：
影响因子：
64.5
通讯作者：
D. Baltimore
中科院分区：
文献类型：
--
作者：
A. Winoto;D. Baltimore
表达al3或yS抗原受体(TCR)的T细胞是不同的细胞谱系。编码 TCR a 和 6 基因的单基因座需要特殊调节以避免基因在 y6 T 细胞中表达。我们在此表明，最小的 a 增强子在 y6 T 细胞谱系中具有活性，但通过 Ca 基因的负作用元件 3' 获得 af3 谱系特异性，该元件使 $3 T 细胞中的增强子沉默。 Ca 基因座的负元件由多个消音器组成，这些消音器以与方向和距离无关的方式工作。这些沉默子还作用于通常普遍表达的逆转录病毒增强子，将其活性限制在 afl 细胞中。 a 沉默子在非 T 细胞系中活跃，表明细胞分化为 aj3 T 细胞谱系的决定可能涉及这些沉默子的特异性缓解。在许多系统中建立谱系特异性基因表达方面，沉默子可能与增强子一样重要。
T cells expressing either the al3 or yS antigen receptor (TCR) are distinct cell lineages. The single locus encoding the TCR a and 6 genes requires special regulation to avoid a gene expression in y6 T ceils. We show here that the minimal a enhancer is active in the y6 T cell lineage but gains af3 lineage specificity through negative &-acting elements 3’of the Ca gene that silence the enhancer in $3 T cells. The negative elements at the Ca locus consist of several silencers that work in an orientation- and distance-independent fashion. These silencers also act on a retmviral enhancer that is normally ubiquitously expressed, restricting its activity to afl cells. The a silencers are active in non-T cell lines, suggesting that the decision of a cell to differentiate into the aj3 T cell lineage may involve specific relief from these silencers. Silencers are likely to be as important as enhancers in establishing lineage-specific gene expression in many systems.