Photostable lysosomal imaging of living cell with hyperspectral stimulated Raman scattering microscopy using a probe based on bisarylbutadiyne
Photostable lysosomal imaging of living cell with hyperspectral stimulated Raman scattering microscopy using a probe based on bisarylbutadiyne
复制标题
使用基于双芳基丁二炔的探针通过高光谱受激拉曼散射显微镜对活细胞进行耐光溶酶体成像
DOI：
10.1016/j.cclet.2019.03.046
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发表时间：
2019-07-01
影响因子：
9.1
通讯作者：
Zhou, Xiang
中科院分区：
文献类型：
--
作者：
Ding, Cong;Chen, Yage;Zhou, Xiang
我们设计了一种溶酶体选择性拉曼探针，将双苯基丁二炔与吗啉偶联，吗啉是一种众所周知的溶酶体靶向基团。这种名为Lyso-Bady的探针的拉曼峰强度是5-乙炔基-2‘-脱氧尿苷的28倍。用这种胞外探针成功地用高光谱受激拉曼散射(SRS)显微镜显示了活细胞中的溶酶体。进一步的研究表明，在延长辐照时间后，Lyso-Bady的拉曼信号仍然稳定而强烈。Lyso-Bady的光稳定性使结构相似的分子成为未来连续成像的潜在通用探针(C)2019中国化学会和中国医学科学院药物研究所。爱思唯尔出版，版权所有。
We designed a lysosome-selective Raman probe by conjugating bisphenylbutadiyne with morpholine, a well-known lysosome targeting moiety. This probe, named Lyso-BADY, has a Raman peak 28 times more intense than that of 5-ethynyl-2'-deoxyuridine. Lysosome in living cells was successfully visualized by hyperspectral stimulated Raman scattering (SRS) microscopy with this extracellular probe. Further study showed that the Raman signal of Lyso-BADY remained steady and strong even after a prolonged irradiation time. The photo-stability feature of Lyso-BADY rendered molecules of the similar structure as potentially versatile probe for continuous imaging in the future (C) 2019 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.