Genetic analysis of tumorigenesis: V. Chromosomal analysis of tumorigenic and nontumorigenic diploid chinese hamster cell lines
Genetic analysis of tumorigenesis: V. Chromosomal analysis of tumorigenic and nontumorigenic diploid chinese hamster cell lines
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肿瘤发生的遗传分析：五、致瘤和非致瘤二倍体中国仓鼠细胞系的染色体分析
DOI：
10.1007/bf01538697
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
R. Sager
中科院分区：
文献类型：
--
作者：
R. Kitchin;R. Sager
本文描述了四个已建立的二倍体中国仓鼠胚胎成纤维细胞（CHEF）细胞系的染色体组成。CHEF/18在裸鼠中表现出锚定依赖性生长并且不具有致瘤性。CHEF/16在甲基纤维素中具有高的接种效率，并且在裸鼠中具有高度致瘤性。CHEF/18和CHEF/16均具有正常二倍体中国仓鼠细胞的正常Giemsa带型和组成性异染色质分布特征，并且在其细胞群体内表现出相对较少的染色体变异。这些结果表明，负责CHEF/16的致瘤性的核变化涉及低于Giemsa显带分析可检测的水平的改变。在另外两个CHEF细胞系CHEF/205-30（CHEF/18的二倍体硫代鸟嘌呤抗性衍生物）和CHEF/204-Bu 50（CHEF/16的二倍体5-溴脱氧尿苷抗性衍生物）中检测到染色体重排，这两个细胞系被用作种内体细胞杂种的遗传标记。
The chromosomal constitution of four established diploid Chinese hamster embryo fibroblast (CHEF) cell lines is described. CHEF/18 exhibits anchorage-dependent growth and is not tumorigenic in nude mice. CHEF/16 has a high plating efficiency in methylcellulose and is highly tumorigenic in nude mice. Both CHEF/18 and CHEF/16 have a normal Giemsa banding pattern and constitutive heterochromatin distribution characteristic of normal diploid Chinese hamster cells and exhibit relatively little chromosomal variation within their cell populations. These results suggest that the nuclear changes responsible for tumorigenicity of CHEF/16 involve alterations below the level detectable by Giemsa banding analysis. Chromosome rearrangements were detected in two other CHEF cell lines, CHEF/205-30, a diploid, thioguanine-resistant derivative of CHEF/18, and CHEF/204-Bu 50, a diploid, 5-bromodeoxyuridine-resistant derivative of CHEF/16, which are being used as genetic markers in intraspecific somatic cell hybrids.