Acidithiobacillus thiooxidans secretome containing a newly described lipoprotein Licanantase enhances chalcopyrite bioleaching rate.
Acidithiobacillus thiooxidans secretome containing a newly described lipoprotein Licanantase enhances chalcopyrite bioleaching rate.
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DOI:
10.1007/s00253-010-3063-8
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发表时间:
2011-02
影响因子:
5
通讯作者:
Parada P
中科院分区:
文献类型:
--
作者:
Bobadilla Fazzini RA;Levican G;Parada P
The nature of the mineral–bacteria interphase where electron and mass transfer processes occur is a key element of the bioleaching processes of sulfide minerals. This interphase is composed of proteins, metabolites, and other compounds embedded in extracellular polymeric substances mainly consisting of sugars and lipids (Gehrke et al., Appl Environ Microbiol 64(7):2743–2747,). On this respect, despite Acidithiobacilli—a ubiquitous bacterial genera in bioleaching processes (Rawlings, Microb Cell Fact 4(1):13,)—has long been recognized as secreting bacteria (Jones and Starkey, J Bacteriol 82:788–789,; Schaeffer and Umbreit, J Bacteriol 85:492–493,), few studies have been carried out in order to clarify the nature and the role of the secreted protein component: the secretome. This work characterizes for the first time the sulfur (meta)secretome of Acidithiobacillus thiooxidans strain DSM 17318 in pure and mixed cultures with Acidithiobacillus ferrooxidans DSM 16786, identifying the major component of these secreted fractions as a single lipoprotein named here as Licanantase. Bioleaching assays with the addition of Licanantase-enriched concentrated secretome fractions show that this newly found lipoprotein as an active protein additive exerts an increasing effect on chalcopyrite bioleaching rate. The online version of this article (doi:10.1007/s00253-010-3063-8) contains supplementary material, which is available to authorized users.
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DOI:
10.1074/jbc.m805963200
发表时间:
2009-01-30
期刊:
The Journal of biological chemistry
影响因子:
--
作者:
Mavridou DAI;Stevens JM;Goddard AD;Willis AC;Ferguson SJ;Redfield C
通讯作者:
Redfield C
影响因子:
2.6
作者:
Sand, W;Gehrke, T
通讯作者:
Gehrke, T
影响因子:
2.9
作者:
Lee, CH;Tsai, CM
通讯作者:
Tsai, CM
影响因子:
4.4
作者:
Guiliani, N;Jerez, CA
通讯作者:
Jerez, CA
影响因子:
4.2
作者:
Bendtsen JD;Kiemer L;Fausbøll A;Brunak S
通讯作者:
Brunak S