Acidithiobacillus thiooxidans secretome containing a newly described lipoprotein Licanantase enhances chalcopyrite bioleaching rate.

Acidithiobacillus thiooxidans secretome containing a newly described lipoprotein Licanantase enhances chalcopyrite bioleaching rate.
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DOI:
10.1007/s00253-010-3063-8
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发表时间:
2011-02
影响因子:
5
通讯作者:
Parada P
Parada P
中科院分区:
工程技术2区
文献类型:
--
作者:
Bobadilla Fazzini RA;Levican G;Parada P

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矿物-细菌界面发生电子和质量传递过程的性质是硫化矿物生物浸出过程的关键因素。该中间相由蛋白质、代谢物和包埋在主要由糖和脂质组成的细胞外聚合物中的其他化合物组成(Gehrke等人,Appl Environ Microbiol 64(7):2743-2747)。在这方面,尽管嗜酸硫杆菌(Acidithiobacillia)--一种在生物浸提过程中普遍存在的细菌属(Rawlings,Microb Cell Fact 4(1):13)--长期以来被认为是分泌细菌(Jones和Starkey,J Bacteriol 82:788-789; Schaeffer和Umbreit,J Bacteriol 85:492-493),但很少进行研究以阐明分泌蛋白组分:分泌体的性质和作用。这项工作的特点,第一次的硫(Meta)分泌组的嗜酸氧化硫硫杆菌菌株DSM 17318在纯的和混合的培养物嗜酸氧化亚铁硫杆菌DSM 16786,确定这些分泌馏分的主要成分作为一个单一的脂蛋白命名为Licanantase在这里。加入富含Licanantase的浓缩分泌体组分的生物浸出测定表明,这种新发现的脂蛋白作为活性蛋白添加剂对黄铜矿生物浸出率产生增加的影响。本文的在线版本(doi:10.1007/s 00253 -010-3063-8)包含补充材料,可供授权用户使用。
The nature of the mineral–bacteria interphase where electron and mass transfer processes occur is a key element of the bioleaching processes of sulfide minerals. This interphase is composed of proteins, metabolites, and other compounds embedded in extracellular polymeric substances mainly consisting of sugars and lipids (Gehrke et al., Appl Environ Microbiol 64(7):2743–2747,). On this respect, despite Acidithiobacilli—a ubiquitous bacterial genera in bioleaching processes (Rawlings, Microb Cell Fact 4(1):13,)—has long been recognized as secreting bacteria (Jones and Starkey, J Bacteriol 82:788–789,; Schaeffer and Umbreit, J Bacteriol 85:492–493,), few studies have been carried out in order to clarify the nature and the role of the secreted protein component: the secretome. This work characterizes for the first time the sulfur (meta)secretome of Acidithiobacillus thiooxidans strain DSM 17318 in pure and mixed cultures with Acidithiobacillus ferrooxidans DSM 16786, identifying the major component of these secreted fractions as a single lipoprotein named here as Licanantase. Bioleaching assays with the addition of Licanantase-enriched concentrated secretome fractions show that this newly found lipoprotein as an active protein additive exerts an increasing effect on chalcopyrite bioleaching rate. The online version of this article (doi:10.1007/s00253-010-3063-8) contains supplementary material, which is available to authorized users.
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