Cyclooxygenase-1 is a marker for a subpopulation of putative nociceptive neurons in rat dorsal root ganglia
Cyclooxygenase-1 is a marker for a subpopulation of putative nociceptive neurons in rat dorsal root ganglia
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DOI：
10.1046/j.1460-9568.2000.00979.x
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发表时间：
2000-03-01
影响因子：
3.4
通讯作者：
Grubb, BD
中科院分区：
文献类型：
--
作者：
Chopra, B;Giblett, S;Grubb, BD
使用免疫细胞化学和形态测量技术来量化大鼠 L5 背根神经节 (DRG) 中含有环氧合酶 (cox) 的神经元的分布。 Cox-1免疫标记几乎完全局限于小直径DRG神经元（<1000μm（2）），并且广泛与降钙素基因相关肽（CGRP）和异凝集素B4（IB4）共定位。 Cox-1 分别存在于 65% 和 70% 的 CGRP 和 IB4 标记的神经元中。在表达感觉神经元特异性 (SNS) Na+ 通道的神经元中也发现了 Cox-1 标记。正常大鼠的 DRG 中不存在 Cox-2 标记。在单关节炎弗氏佐剂模型中，cox-1阳性DRG神经元的比例没有变化，并且没有发现被cox-2标记的神经元。在原代组织培养中，cox-1 免疫标记在体外可持续长达 9 天，并且存在于形态相同的神经元中。少量标记为 cox-1 的结状神经节神经元和颈上神经节 (SCG) 神经元证实了 cox-1 在外周神经节中的选择性表达。这些数据表明，cox-1 是体外和体内假定的伤害性神经元亚群的标记，并表明这些神经元合成的前列腺素可能对伤害性感受器功能很重要。这些数据可能对非甾体抗炎药（NSAID）的作用方式和机制具有重要意义。
Immunocytochemical and morphometric techniques were used to quantify the distribution of cyclooxygenase (cox)-containing neurons in rat L5 dorsal root ganglia (DRG). Cox-1 immunolabelling was almost exclusively restricted to small diameter DRG neurons (< 1000 mu m(2)), and was extensively colocalized with calcitonin gene-related peptide (CGRP) and isolectin B4 (IB4). Cox-1 was present in 65% and 70% of CGRP- and IB4-labelled neurons, respectively. Cox-1 labelling was also found in neurons expressing the sensory neuron-specific (SNS) Na+ channel. Cox-2 labelling was absent in DRG from normal rats. In the Freund's adjuvant model of monoarthritis, the proportion of cox-1-positive DRG neurons was unchanged and no neurons were found to be labelled for cox-2. In primary tissue culture, cox-1 immunolabelling persisted in vitro for up to 9 days and was present in morphologically identical neurons. The selective expression of cox-1 in peripheral ganglia was confirmed by the small number of nodose ganglion neurons and superior cervical ganglion (SCG) neurons labelled for cox-1. These data suggest that cox-1 is a marker for a subpopulation of putative nociceptive neurons in vitro and in vivo, and suggests that the prostaglandins synthesized by these neurons may be important for nociceptor function. These data may have important implications for the mode and mechanism of action of non-steroidal anti-inflammatory drugs (NSAIDs).