Latex immunoassay of retinol-binding protein.
Latex immunoassay of retinol-binding protein.
复制标题
视黄醇结合蛋白的乳胶免疫测定。
DOI：
10.1093/clinchem/28.5.1167
复制
发表时间：
1982
影响因子：
9.3
通讯作者：
R. Lauwerys
中科院分区：
文献类型：
--
作者：
A. Bernard;D. Moreau;R. Lauwerys
胶乳免疫分析法是一种基于胶乳颗粒凝集的灵敏方法，用于测定人体体液中的视黄醇结合蛋白。该测定法与先前描述的β 2-微球蛋白测定法（Clin.Chem.27：832-837，1981）相似，包括将样品与抗体包被颗粒在37 ℃下孵育30 min，然后通过颗粒计数或比浊法定量所得凝集。用抗体包被的乳胶颗粒在临使用前通过将它们分散在pH 10的牛血清白蛋白溶液中来稳定。标准曲线范围为0.5 - 32微克/升;尿液和血清中的平均回收率分别为102%和93.5%;试验间和试验内CV范围为5.1 - 11.7%。26份尿液和30份血清中视黄醇结合蛋白的乳胶免疫测定与火箭免疫电泳法和放射免疫扩散法的相关系数分别为0.99和0.91。在健康受试者中，视黄醇结合蛋白的平均尿排泄量为52.5微克/克肌酐（SD = 59.2微克/克肌酐; n = 150），血清浓度平均为46 mg/L（SD = 10.4 mg/L，n = 22）。
Latex immunoassay, a sensitive method based on latex particle agglutination, is used here for the determination of retinol-binding protein in human biological fluids. The assay, similar to that described previously for beta 2-microglobulin (Clin. Chem. 27:832-837, 1981), consists of incubating the sample at 37 degrees C for 30 min with antibody-coated particles, then quantifying the resulting agglutination by particle-counting or turbidimetry. Latex particles coated with antibody are stabilized just before use by dispersing them in a solution of bovine serum albumin at pH 10. The standard curve ranges from 0.5 to 32 micrograms/L; recovery averages 102% in urine and 93.5% in serum; between- and within-assay CVs range from 5.1 to 11.7%. The correlation coefficients of latex immunoassay with rocket immunoelectrophoresis for analysis of retinol-binding protein in 26 urines and with radial immunodiffusion in 30 sera are 0.99 and 0.91, respectively. In healthy subjects, the mean urinary excretion of retinol-binding protein is 52.5 micrograms/g of creatinine (SD = 59.2 micrograms/g of creatinine; n = 150) and the concentration in serum averages 46 mg/L (SD = 10.4 mg/L, n = 22).