A facile voltammetric method for detection of heparin in plasma based on polyethylenimine modified electrode
A facile voltammetric method for detection of heparin in plasma based on polyethylenimine modified electrode
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基于聚乙烯亚胺修饰电极的简便伏安法检测血浆中肝素
DOI：
10.1039/c8ay02722f
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发表时间：
2019
影响因子：
3.1
通讯作者：
Zipin Zhang
中科院分区：
文献类型：
--
作者：
Lei Tian;Hongsu Zhao;Zuzhi Zhao;Jiali Zhai;Zipin Zhang
开发一种技术简单但有效的血浆中肝素定量方法具有重要意义，因为它具有高度的生物学和临床相关性。本研究展示了一种基于聚乙烯亚胺 (PEI) 修饰电极的简便且经济有效的伏安法，用于检测血浆中的肝素。在此，PEI通过碳纳米管固定在电极表面上，然后由于Fe(CN)63−和PEI之间的静电相互作用，氧化还原探针铁氰化物（即Fe(CN)63−）被限制。肝素的存在导致 Fe(CN)63− 从电极表面去除，因为 PEI 对肝素的亲和力高于对 Fe(CN)63− 的亲和力，这验证了使用限制 Fe(CN)63− 的电流减少作为信号读数的肝素检测。该方法显示肝素的动态线性浓度在 0.1–2 U mL−1 和 2–20 U mL−1 范围内，涵盖临床相关的肝素水平。动物实验证明该方法对血浆中肝素水平的定量具有高度选择性和相当有效。本研究为肝素检测提供了一种简便的伏安法，技术要求较低，操作程序简单，有望找到更多与肝素相关的生物学和临床应用。
The development of a technically simple yet effective method for the quantification of heparin in plasma is of great importance because of its high biological and clinical relevance. This study demonstrates a facile and cost-effective voltammetric method for detection of heparin in plasma based on the polyethylenimine (PEI) modified electrode. Herein, PEI was immobilized onto the electrode surface via carbon nanotubes and the redox probe ferricyanide (i.e., Fe(CN)63−) was afterwards confined owing to the electrostatic interaction between Fe(CN)63− and PEI. The presence of heparin caused Fe(CN)63− to be removed from the electrode surface because of the higher affinity of PEI toward heparin than toward Fe(CN)63−, which validates heparin detection using the current decrease of confined Fe(CN)63− as the signal readout. The method shows dynamic linear concentration of heparin in the ranges of 0.1–2 U mL−1 and 2–20 U mL−1 which cover the clinically relevant levels of heparin. With the animal experiments, the method has been demonstrated to be highly selective and quite effective for heparin level quantification in plasma. This study offers a facile voltammetric method for heparin detection with less technically demanding and simple operating procedures, which could be envisaged to find more biological and clinical applications associated with heparin.