Suppression of the tumorigenic phenotype in human oral squamous cell carcinoma cells by an ethanol extract derived from freeze-dried black raspberries
Suppression of the tumorigenic phenotype in human oral squamous cell carcinoma cells by an ethanol extract derived from freeze-dried black raspberries
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DOI：
10.1207/s15327914nc5401_7
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发表时间：
2006-01-01
影响因子：
2.9
通讯作者：
Mallery, Susan R.
中科院分区：
文献类型：
--
作者：
Rodrigo, Kapila A.;Rawal, Yeshwant;Mallery, Susan R.
尽管集中努力改善治疗，5年生存率的人与晚期口腔鳞状细胞癌（SCC）仍然令人沮丧的低。显然，早期检测与局部干预策略相结合，例如在SCC发生之前进行化学预防，可以显着改善临床结果。然而，先前进行的口腔人类化学预防试验提供了混合的结果。虽然一些治疗显示出有效性，但它们通常伴随着显著的毒性或循环抗腺病毒抗体。很明显，确定无毒，有效的治疗是必不可少的，以防止口腔上皮发育不良的恶性转化。本研究采用从人口腔SCC肿瘤中分离的细胞系，研究冻干黑树莓乙醇提取物（RO-ET）对细胞生长特性的影响，这些细胞生长特性通常与转化表型相关，如持续增殖、诱导血管生成和产生高水平的反应性物质。我们的研究结果表明，RO-ET抑制细胞增殖而不干扰生存能力，抑制翻译的完整的血管生成细胞因子血管内皮生长因子抑制一氧化氮合酶的活性，并诱导细胞凋亡和终末分化。这些数据表明，RO-ET是一个有前途的候选人作为一种化学预防剂的人与口腔上皮发育不良。
Despite focused efforts to improve therapy, 5-yr survival rates for persons with advanced-stage oral squamous cell carcinoma (SCC) remain discouragingly low. Clearly, early detection combined with strategies for local intervention, such as chemoprevention prior to SCC development, could dramatically improve clinical outcomes. Previously conducted oral cavity human chemoprevention trials, however have provided mixed results. Although some therapies showed efficacy, they were often accompanied by either significant toxicities or circulating antiadenoviral antibodies. It is clearly apparent that identification of nontoxic, effective treatments is essential to prevent malignant transformation of oral epithelial dysplasias. This study employed cell lines isolated from human oral SCC tumors to investigate the effects of a freeze-dried black raspberry ethanol extract (RO-ET) on cellular growth characteristics often associated with a transformed phenotype such as sustained proliferation, induction of angiogenesis, and production of high levels of reactive species. Our results demonstrate that RO-ET suppresses cell proliferation without perturbing viability, inhibits translation of the complete angiogenic cytokine vascular endothelial growth factor suppresses nitric oxide synthase activity, and induces both apoptosis and terminal differentiation. These data imply that RO-ET is a promising candidate for use as a chemopreventive agent in persons with oral epithelial dysplasia.