On-tissue identification of insulin: in situ reduction coupled with mass spectrometry imaging.
On-tissue identification of insulin: in situ reduction coupled with mass spectrometry imaging.
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胰岛素的组织鉴定：原位还原与质谱成像结合。
DOI：
10.1002/prca.201000152
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发表时间：
2011-08
影响因子：
2
通讯作者：
Nyalwidhe, Julius O.
中科院分区：
文献类型：
--
作者：
Green-Mitchell, Shamina M.;Cazares, Lisa H.;Semmes, O. John;Nadler, Jerry L.;Nyalwidhe, Julius O.
The aim of this study was to use on- tissue reduction followed by MALDI-IMS to identify an m/z 5812.85 peak which is over-expressed in healthy human pancreatic tissue compared to Type one Diabetes (T1D) tissue. A major constraint of MALDI-IMS is identification of compounds with m/z ≥ 4000. On-tissue reduction using tris (2-carboxyethyl) phosphine (TCEP) breaks the inter-domain disulphide bonds generating low molecular weight peptides amenable to direct MS/MS analysis. Pancreatic tissues from healthy (n=1) and diabetic subjects (n=1) were profiled by MALDI-IMS with/without reduction. On-tissue reduction resulted in the loss of the over expressed 5812.85 m/z peak and the simultaneous appearance of a 3430.664 m/z peak in healthy tissue. The latter peak presumably derived from the 5812.85 m/z peak was identified as the insulin B chain by MS/MS. MALDI-IMS images show that both the 5812.85 insulin peak before reduction and the 3430.664 peak after reduction co-localized with the healthy pancreatic islets. On-tissue reduction followed by MALDI-IMS resulted in the identification of insulin and localization of pancreatic islets of langerhans. The approach will be useful in the future identification of novel therapeutic molecular targets to beta-cells lost during T1D.
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影响因子：
4.3
作者：
Mormann, Michael;Eble, Johannes;Pohlentz, Gottfried
通讯作者：
Pohlentz, Gottfried
影响因子：
64.8
作者：
MATSUMURA, M;SIGNOR, G;MATTHEWS, BW
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MATTHEWS, BW
影响因子：
4.3
作者：
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Holle, A
影响因子：
2.3
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通讯作者：
Caprioli, Richard M.
影响因子：
7.4
作者：
Caprioli, RM;Farmer, TB;Gile, J
通讯作者：
Gile, J