Genetic heterogeneity of Escherichia coli isolated from pasteurized milk in State of Paraná, Brazil
Genetic heterogeneity of Escherichia coli isolated from pasteurized milk in State of Paraná, Brazil
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DOI：
10.1590/s1984-82502014000200013
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发表时间：
2014-06-01
影响因子：
1.3
通讯作者：
Mikcha, Jane Martha Graton
中科院分区：
文献类型：
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作者：
Oltramari, Karine;Cardoso, Rosilene Fressati;Mikcha, Jane Martha Graton
肠道病原体引起的食品污染是全世界腹泻病的主要原因，导致高发病率和死亡率以及巨大的经济损失。细菌是食源性疾病的重要病原体，特别是致腹泻性大肠杆菌。本研究评估了巴西西北部巴拉那州 21 家乳制品厂加工的巴氏灭菌牛奶中分离出的大肠杆菌的遗传多样性和抗菌药物耐药性。根据临床和实验室标准研究所的建议，对 95 株大肠杆菌分离株进行了抗菌药敏测试，并通过肠杆菌重复基因间一致性聚合酶链反应 (ERIC-PCR) 进行基因型评估。头孢噻吩的耐药率最高（55.78%）。 ERIC-PCR 显示出高度的遗传多样性，将 95 种细菌分离株聚类成 90 种不同的基因型模式。这些结果表明，巴拉那州西北部地区生产的牛奶样品中存在不同程度的大肠杆菌，并且在巴氏灭菌牛奶的整个加工过程中需要采用良好的生产规范，以降低食源性疾病的风险。
Food contamination caused by enteric pathogens is a major cause of diarrheal disease worldwide, resulting in high morbidity and mortality and significant economic losses. Bacteria are important agents of foodborne diseases, particularly diarrheagenic Escherichia coli. The present study assessed the genetic diversity and antimicrobial resistance of E. coli isolates from pasteurized milk processed in 21 dairies in northwestern State of Parana, Brazil. The 95 E. coli isolates were subjected to antimicrobial susceptibility testing according to the recommendations of the Clinical and Laboratory Standards Institute and assessed genotypically by Enterobacterial Repetitive Intergenic Consensus-Polymerase Chain Reaction (ERIC-PCR). The highest rate of resistance was observed for cephalothin (55.78%). ERIC-PCR revealed high genetic diversity, clustering the 95 bacterial isolates into 90 different genotypic patterns. These results showed a heterogeneous population of E. coli in milk samples produced in the northwestern region of Parana and the need for good manufacturing practices throughout the processing of pasteurized milk to reduce the risk of foodborne illnesses.