Identification of Helicobacter pylori biofilms in human gastric mucosa
Identification of Helicobacter pylori biofilms in human gastric mucosa
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DOI：
10.1016/j.gassur.2005.10.019
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发表时间：
2006-05-01
影响因子：
3.2
通讯作者：
Coticchia, James M.
中科院分区：
文献类型：
--
作者：
Carron, Michael A.;Tran, Vivian R.;Coticchia, James M.
本研究的目的是使用内镜下直接活检和扫描电子显微镜（SEM）来记录幽门螺杆菌生物膜在人胃粘膜中的存在。患者接受了柔性食管胃内窥镜检查，并进行了三次胃粘膜活检。进行快速尿素酶检测以确定是否存在幽门螺杆菌。用SEM对尿素酶阳性和尿素酶阴性对照标本进行成像，以获得胃粘膜的详细图像，用于鉴定生物膜菌落。样本来自接受食管胃镜检查的患者。11例幽门螺杆菌阳性，9例幽门螺杆菌阴性。这些用电子显微镜在500 X和1000 X下成像。幽门螺杆菌阳性标本的细胞表面存在致密、成熟的生物膜，而尿素酶阴性对照中则不存在。获得显微照片。生物膜是一种复杂的微生物生态系统，其中固着细菌将自身包围在保护性基质中。这种生活方式提供保护，允许在恶劣环境中生长，并改变宿主的生理机能。许多人假设幽门螺杆菌感染导致胃溃疡可能是生物膜的一种表现。我们的研究是第一个实验性地证明人胃粘膜上存在幽门螺杆菌生物膜。这种生物体粘膜的生态学和病理生理学的阐明对于我们理解这种生物体对当前治疗的抗性的潜在机制以及如何在未来更好地根除它是重要的。
The purpose of this study was to use endoscopically directed biopsies and scanning electron microscopy (SEM) to document the existence of Helicobacter pylori biofilms in human gastric mucosa. Patients underwent flexible esophagogastroduodenoscopies with three gastric mucosal biopsies. Rapid urease testing was performed to determine the presence or absence of H pylori. Urease-positive and urease-negative control specimens were imaged with SEM to obtain detailed images of gastric mucosa for the identification of biofilm colonies. Samples were obtained from patients who under-went esophagogastroduodenoscopies. Eleven were found to be H pylori positive and nine were H pylori negative. These were imaged at 500X and 1000X with electron microscopy. Dense, mature biofilms were present and attached to the cell surface of H pylori-positive specimens and were absent in urease-negative controls. Photomicrographs were obtained. Biofilms are complex microbiological ecosystems where sessile bacteria surround themselves in a protective matrix. This lifestyle affords protection, allows for growth in hostile environments, and alters host physiology. Many have hypothesized that H pylori infections resulting in gastric ulcers may be a manifestation of biofilms. Our investigation is the first to photographically document the existence of H pylori biofilms on human gastric mucosa. This elucidation of the ecology and pathophysiology of the mucosa of the organism is important to our understanding of a potential mechanism of this organism's resistance to current therapy and how to better eradicate it in the future.