Differential effects of adrenocorticotropin(1-24) on [3H]2-deoxy-D-glucose uptake in cultured cells derived from different brain regions.
Differential effects of adrenocorticotropin(1-24) on [3H]2-deoxy-D-glucose uptake in cultured cells derived from different brain regions.
复制标题
促肾上腺皮质激素 (1-24) 对来自不同脑区的培养细胞中 [3H]2-脱氧-D-葡萄糖摄取的不同影响。
DOI：
10.1016/0304-3940(88)90016-x
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发表时间：
1988
影响因子：
2.5
通讯作者：
Kapcala,LP
中科院分区：
文献类型：
--
作者：
Bistritzer,T;Levin,PA;Roeder,LM;Kapcala,LP
本文比较了促肾上腺皮质激素（1-24）（ACTH（1-24））对胎鼠下丘脑和下丘脑外前脑细胞摄取[3 H] 2-脱氧-D-葡萄糖（[3 H] 2-DG）的影响。在对照条件下，下丘脑外（10.9±1.1 nmol mg蛋白5 min）和下丘脑（11.9± 1.3 nmol mg蛋白5 min）细胞摄取[3 H] 2-DG相似。未发现ACTH（1-24）（10− 7至10− 5 M）对下丘脑外细胞摄取[3 H] 2-DG有显著影响。与此相反，在下丘脑细胞中，这些浓度的ACTH（1-24）产生了强刺激作用（P< 0.0001），超过对照值的[3 H] 2-DG摄取高达174%。这项研究表明，促肾上腺皮质激素可能是脑葡萄糖摄取的刺激剂，这种作用在不同的脑区不同。
Abstract The effect of adrenocorticotropin (1–24)(ACTH (1–24)) on the uptake of [3 H] 2-deoxy-d-glucose ([3 H] 2-DG) was compared in cell cultures derived from two regions (hypothalamus, and extrahypothalamic forebrain) of fetal rat brain. Under control conditions,[3 H] 2-DG uptake was similar in extrahypothalamic (10.9±1.1 nmol mg protein 5 min) and hypothalamic (11.9±1.3) cells. No significant effect of ACTH (1–24)(10− 7 to 10− 5 M) was found on uptake of [3 H] 2-DG in extrahypothalamic cells. In contrast, in hypothalamic cells, a potent stimulatory effect (P< 0.0001) up to 174% over the control value of [3 H] 2-DG uptake was produced by these concentrations of ACTH (1–24). This study suggests that ACTH may be a stimulator of brain glucose uptake, and that this effect varies in different brain regions.