The Vibrio motor proteins, MotX and MotY, are associated with the basal body of Na+-driven flagella and required for stator formation
The Vibrio motor proteins, MotX and MotY, are associated with the basal body of Na+-driven flagella and required for stator formation
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DOI：
10.1111/j.1365-2958.2006.05435.x
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发表时间：
2006-11-01
影响因子：
3.6
通讯作者：
Homma, Michio
中科院分区：
文献类型：
--
作者：
Terashima, Hiroyuki;Fukuoka, Hajime;Homma, Michio
四种运动蛋白PomA、PomB、mox和MotY被认为是定子蛋白，它们是溶藻弧菌鞭毛运动所必需的Na+驱动蛋白。纯化鞭毛基体时，在基体中检测到由转子和衬套组成的超分子复合物mox和MotY，但未检测到PomA和PomB。通过抗体标记，在LP环周围检测到MotX和MotY。这些结果表明，mox和MotY与基底体有关。基底在LP环下形成新的环状结构，命名为T环。在Delta mox或Delta motY应变中，基底的这种结构发生了改变或消失。T环可能由mox和MotY组成。在缺乏mox或MotY的情况下，我们证明了PomA和PomB不定位于细胞极点。从上述结果来看，我们认为T环的mox和MotY参与了电机中PomA/PomB复合物的结合和/或稳定。
The four motor proteins PomA, PomB, MotX and MotY, which are believed to be stator proteins, are essential for motility by the Na+-driven flagella of Vibrio alginolyticus. When we purified the flagellar basal bodies, MotX and MotY were detected in the basal body, which is the supramolecular complex comprised of the rotor and the bushing, but PomA and PomB were not. By antibody labelling, MotX and MotY were detected around the LP ring. These results indicate that MotX and MotY associate with the basal body. The basal body had a new ring structure beneath the LP ring, which was named the T ring. This structure was changed or lost in the basal body from a Delta motX or Delta motY strain. The T ring probably comprises MotX and MotY. In the absence of MotX or MotY, we demonstrated that PomA and PomB were not localized to a cell pole. From the above results, we suggest that MotX and MotY of the T ring are involved in the incorporation and/or stabilization of the PomA/PomB complex in the motor.