Pentagastrin selectively modulates levels of mRNAs encoding apical H/K adenosine triphosphatase and basolateral Na-K-Cl cotransporter in rat gastric fundic mucosa.
Pentagastrin selectively modulates levels of mRNAs encoding apical H/K adenosine triphosphatase and basolateral Na-K-Cl cotransporter in rat gastric fundic mucosa.
复制标题
五胃泌素选择性调节大鼠胃底粘膜中编码顶端 H/K 腺苷三磷酸酶和基底外侧 Na-K-Cl 协同转运蛋白的 mRNA 水平。
DOI：
10.1016/s0039-6060(96)80294-7
复制
发表时间：
1996
期刊：
影响因子：
3.8
通讯作者：
Soybel,DI
中科院分区：
文献类型：
--
作者：
Klingensmith,ME;Hallonquist,H;McCoy,BP;Cima,RR;Delpire,E;Soybel,DI
背景胃泌素调节胃酸分泌和胃粘膜细胞增殖。我们假设，五肽胃泌素管理会影响mRNA水平的两个膜蛋白，这是重要的，在刺激状态下的盐酸分泌，基底外侧Na−K−Cl协同转运蛋白（BSC）和顶端H/K腺苷三磷酸酶（H/K）MethodsTwo groups of Fischer rats received injections of pentagastrin（2.5或25 μg/kg），每8小时为三个剂量。第三组作为对照组。另一组接受五肽胃泌素加胃泌素受体拮抗剂（GRA）L740，093。对胃底粘膜进行编码H/K和BSC的mRNA的半定量北方分析。Na/K腺苷三磷酸酶（Na/K），运输蛋白不直接参与酸分泌，也evaluated.ResultsAdministration的五肽胃泌素引起的mRNA编码H/K和BSC的水平呈剂量依赖性增加，但Na/K mRNA的水平没有显着的影响。GRA的管理防止pentagastrin诱导的mRNA水平的变化，这些transporters.ConclusionsPentagastrin管理选择性上调参与酸分泌的膜蛋白的mRNA编码水平。在五肽胃泌素刺激过程中编码BSC的mRNA的上调表明，在刺激状态下，基底外侧Cl−运动的调节可能与顶端H+运动的调节一样重要。
BackgroundGastrin regulates gastric acid secretion and gastric mucosal cell proliferation. We hypothesized that pentagastrin administration would affect mRNA levels of two membrane proteins that are important during stimulated states of HCl secretion, the basolateral Na−K−Cl cotransporter (BSC) and the apical H/K adenosine triphosphatase (H/K).MethodsTwo groups of Fischer rats received intraperitoneal injections of pentagastrin (2.5 or 25 μg/kg) every 8 hours for three doses. A third group served as controls. An additional group receeved pentagastrin plus the gastrin receptor antagonist (GRA) L740,093. Fundic mucosae were subjected to semiquantitative Northern analysis of mRNAs encoding H/K and BSC. The mRNA for Na/K adenosine triphosphatase (Na/K), a transport protein not involved directly in acid secretion, also was evaluated.ResultsAdministration of pentagastrin caused dose-dependent increases in levels of mRNAs encoding H/K and BSC but had no significant effect on levels of Na/K mRNA. Administration of GRA prevented the pentagastrin-induced changes in mRNA levels for these transporters.ConclusionsPentagastrin administration selectively up-regulates levels of mRNA encoding membrane proteins involved in acid secretion. The up-regulation of the mRNAs encoding BSC during pentagastrin stimulation indicates that regulation of basolateral Cl−movement may be as important as the regulation of apical H+movement under stimulated states.