AEROBIC VINYL-CHLORIDE METABOLISM IN MYCOBACTERIUM-AURUM L1
AEROBIC VINYL-CHLORIDE METABOLISM IN MYCOBACTERIUM-AURUM L1
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DOI：
10.1128/aem.58.4.1220-1226.1992
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发表时间：
1992-04-01
影响因子：
4.4
通讯作者：
DEBONT, JAM
中科院分区：
文献类型：
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作者：
HARTMANS, S;DEBONT, JAM
金分枝杆菌L1，能够生长在氯乙烯作为唯一的碳源和能源，以前分离出的土壤污染的氯乙烯（S。Hartmans等人，Biotechnol. Lett. 7：383-388，1985）。 菌株L1中氯乙烯代谢的初始步骤由烯烃单加氧酶催化，将氯乙烯转化为反应性环氧化物氯环氧乙烷。 负责氯环氧乙烷降解的酶似乎是非常不稳定的，从而阻碍了氯乙烯代谢的第二步的表征。 二氯乙烯也被氯乙烯生长的细胞株L1氧化，但它们不被用作生长基质。 从没有已知氯乙烯污染的环境中分离出另外三种利用氯乙烯作为唯一碳源和能源的细菌菌株。 这3株新分离株与L1株相似，也被鉴定为金分枝杆菌。
Mycobacterium aurum L1, capable of growth on vinyl chloride as a sole carbon and energy source, was previously isolated from soil contaminated with vinyl chloride (S. Hartmans et al., Biotechnol. Lett. 7:383-388, 1985). The initial step in vinyl chloride metabolism in strain L1 is catalyzed by alkene monooxygenase, transforming vinyl chloride into the reactive epoxide chlorooxirane. The enzyme responsible for chlorooxirane degradation appeared to be very unstable and thus hampered the characterization of the second step in vinyl chloride metabolism. Dichloroethenes are also oxidized by vinyl chloride-grown cells of strain L1, but they are not utilized as growth substrates. Three additional bacterial strains which utilize vinyl chloride as a sole carbon and energy source were isolated from environments with no known vinyl chloride contamination. The three new isolates were similar to strain L1 and were also identified as Mycobacterium aurum.