Enhanced optical breakdown in KB cells labeled with folate-targeted silver-dendrimer composite nanodevices.
Enhanced optical breakdown in KB cells labeled with folate-targeted silver-dendrimer composite nanodevices.
复制标题
用叶酸靶向银树枝状聚合物复合纳米器件标记的 KB 细胞中的光学击穿增强。
DOI：
10.1016/j.nano.2010.09.003
复制
发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Balogh,Lajos
中科院分区：
文献类型：
--
作者：
Tse,Christine;Zohdy,MarwaJ;Ye,JingYong;O'Donnell,Matthew;Lesniak,Wojciech;Balogh,Lajos
描述了用银-树枝状聚合物复合纳米器件（CND）增强KB肿瘤细胞叶酸靶向的光学击穿。CND [（Ag 0）25-PAMAM_E5.（NH 2）42（NGly）74（NFA）2.7]通过反应性包封，使用树枝状聚合物-叶酸（FA）缀合物的生物相容性模板制备。用共聚焦显微镜和透射电子显微镜观察KB细胞对叶酸靶向CND（各种治疗浓度和表面功能）的优先摄取。细胞内激光诱导的光学击穿阈值和动力学进行检测，其特征在于所产生的瞬态气泡事件的高频超声监测。当用近红外飞秒激光照射时，CND靶向的KB细胞充当激光能量的良好限制的活化剂，增强非线性能量吸收，表现出击穿阈值的显著降低，从而选择性地促进细胞内激光诱导的光学击穿。来自临床编辑：这项研究提出了一种新的方法，选择性地破坏癌细胞相结合的生化靶向局部激光照射。用叶酸银-树枝状聚合物复合纳米器件靶向人表皮样癌细胞系，与未标记的细胞相比，标记的癌细胞随后被由于激光照射的银纳米颗粒的增强的能量吸收而产生的微泡破坏。
Enhanced optical breakdown of KB tumor cells folate-targeted with silver-dendrimer composite nanodevices (CNDs) is described. CNDs [(Ag0)25-PAMAM_E5.(NH2)42(NGly)74(NFA)2.7] were fabricated by reactive encapsulation, using a biocompatible template of dendrimer–folic acid (FA) conjugates. Preferential uptake of the folate-targeted CNDs (of various treatment concentrations and surface functionality) by KB cells was visualized with confocal microscopy and transmission electron microscopy. Intracellular laser-induced optical breakdown threshold and dynamics were detected and characterized by high-frequency ultrasonic monitoring of resulting transient bubble events. When irradiated with a near-infrared, femtosecond laser, the CND-targeted KB cells acted as well-confined activators of laser energy, enhancing nonlinear energy absorption, exhibiting a significant reduction in breakdown threshold and thus selectively promoting intracellular laser-induced optical breakdown. FROM THE CLINICAL EDITOR: This study presents a novel method to selectively destroy cancer cells by combining biochemical targeting with topical laser irradiation. A human epidermoid cancer cell line was targeted with folated silver-dendrimer composite nanodevices and the labeled cancer cells were subsequently destroyed by the microbubbles generated due the enhanced energy uptake of the silver nanoparticles from the laser irradiation, as compared to unlabeled cells.