Allelic and population variation of microsatellite loci in aspen (Populus tremuloides)
Allelic and population variation of microsatellite loci in aspen (Populus tremuloides)
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DOI：
10.1111/j.1469-8137.2005.01423.x
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发表时间：
2005-07-01
期刊：
影响因子：
9.4
通讯作者：
Cole, CT
中科院分区：
文献类型：
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作者：
Cole, CT
为了建立种群遗传和进化的可靠基础，本研究对美国威斯康辛州11个地点的192棵白杨（白杨）基因组16个微卫星位点的等位基因和种群变异进行了分析。毛细管电泳分析荧光标记等位基因的频率分布与重复大小和重复数的关系。将种群水平的统计数据与其他研究进行了比较，特别是杨树。所有的位点都是多态性的，每个位点的等位基因数量差异很大（平均= 8.25，范围2-20）。预期和观察到的杂合度较高（分别为0.45和0.41），种群间分化较小（F-ST = 0.006-0.045），中等近交水平（F-IS = 0.09），在基于同工酶的研究中处于中等水平。与其他一些报道相反，等位基因频率紧密地聚集在模态频率周围，遗传多样性（以每个位点的等位基因或预期的杂合性来衡量）与重复单位大小或重复次数无关。
To develop a robust basis for inferences about population genetics and evolution, this work assayed 192 aspens (Populus tremuloides) from 11 sites in Wisconsin, USA, for allelic and population variation at 16 microsatellite loci distributed across the Populus genome.Frequency distributions of fluorochrome-labeled alleles resolved by capillary electrophoresis were analyzed for relationships to repeat size and number. Population-level statistics were compared with those of other studies, especially in Populus.All loci were polymorphic, varying widely in the number of alleles per locus (mean = 8.25, range 2-20). Expected and observed heterozygosities were high (0.45 and 0.41, respectively), with little differentiation among populations (F-ST = 0.006-0.045) and a moderate level of inbreeding (F-IS = 0.09), intermediate among levels reported in studies based on isozymes.Contrary to several other reports, allele frequencies clustered tightly around the modal frequency, and the genetic diversity (measured as alleles per locus or as expected heterozygosity) was not related to either the repeat unit size or to the number of repeats.