SENSITIVE ASSAY OF HYDROXYL FREE-RADICAL FORMATION UTILIZING HIGH-PRESSURE LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION OF PHENOL AND SALICYLATE HYDROXYLATION PRODUCTS
SENSITIVE ASSAY OF HYDROXYL FREE-RADICAL FORMATION UTILIZING HIGH-PRESSURE LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION OF PHENOL AND SALICYLATE HYDROXYLATION PRODUCTS
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DOI：
10.1016/0165-022x(84)90042-3
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发表时间：
1984-01-01
影响因子：
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通讯作者：
WONG, PK
中科院分区：
文献类型：
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作者：
FLOYD, RA;WATSON, JJ;WONG, PK
本文介绍了一种灵敏的检测各种体系中羟基自由基生成的方法。该方法采用高压液相色谱-电化学检测（LCED）来定量和鉴定来自OH与苯酚和水杨酸盐反应的羟基化产物。羟基化产物的检测限< 1 pmol，电化学检测器响应在至少3个数量级上呈线性。证明了在从生物学上有意义的系统产生OH或OH的过程中获得和形成的羟基化产物的检测和定量。所用的3个系统是ADP/FE（II）/H2 O2、次黄嘌呤/黄嘌呤氧化酶加螯合铁和UV光解H2 O2。
A sensitive method useful for the detection of hydroxyl free radical generation in various systems is described. The methodology employs high pressure liquid chromatography with electrochemical detection (LCED) for the quantification and identification of the hydroxylation products from the reaction of .ovrhdot.OH with both phenol and salicylate. A detection limit of < 1 pmol for the hydroxylation products has been achieved with electrochemical detector responses linear over at least 3 orders of magnitude. Detection and quantitation of the hydroxylation products obtained and formed during .ovrhdot.OH generation from biologically meaningful systems were demonstrated. The 3 systems utilized were ADP/FE(II)/H2O2, hypoxanthine/xanthine oxidase plus chelated iron and UV photolysis of H2O2.