Kinetics of antiviral CD8 T cell responses during primary and post-vaccination secondary bovine respiratory syncytial virus infection
Kinetics of antiviral CD8 T cell responses during primary and post-vaccination secondary bovine respiratory syncytial virus infection
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DOI：
10.1016/j.vaccine.2005.10.005
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发表时间：
2006-03-06
期刊：
影响因子：
5.5
通讯作者：
Van der Most, RG
中科院分区：
文献类型：
--
作者：
Antonis, AFG;Claassen, EAW;Van der Most, RG
我们已经测量了牛呼吸道合胞病毒（bRSV）感染的小牛中的抗病毒CD 8 T细胞应答，这些小牛已经用福尔马林灭活（FI）或减毒活（L）bRSV进行免疫接种，并在用FI-bRSV接种的小牛攻毒后观察到免疫病理学证据。在所有病例中，bRSV感染均诱导了有效的肺CD 8 T细胞应答。与FI-bRSV和PBS组相比，L-bRSV免疫的动物中攻击后应答的动力学加速，表明仅L-bRSV疫苗而非FI-bRSV疫苗具有致敏的记忆T细胞。就肺部CD 8 T细胞的增殖状态而言，初次和接种后继发感染之间的差异非常小。在FI-bRSV接种的动物中，功能性IFN-γ（+）CD 8应答略高。此外，攻击后FI-bRSV接种的动物中存在强IFN-γ（+）CD 8应答表明（i）FI-bRSV免疫的动物中的这些IFN-γ（+）应答不保护免受免疫病理学影响，和（ii）在用FI-bRSV接种后bRSV攻击期间Th-2偏向性应答对支气管肺泡灌洗液中的CD 8应答具有有限的影响。因此，几种反应模式（Th-1/Th-2）似乎在bRSV感染期间共存。(c)2005爱思唯尔有限公司保留所有权利。
We have measured antiviral CD8 T cells responses in bovine respiratory syncytial virus (bRSV) infected calves that had been immunized with either formalin-inactivated (FI) or live-attenuated (L) bRSV, with evidence of immunopathology following challenge of calves vaccinated with FI-bRSV. In all cases, bRSV infection induced potent pulmonary CD8 T cell responses. The kinetics of the post-challenge response in L-bRSV immunized animals was accelerated compared to the FI-bRSV and PBS groups, suggesting that only the L-bRSV vaccine, and not the FI-bRSV vaccine, had primed memory T cells. The differences between primary and post-vaccination secondary infection were very minor, in terms of the proliferation status of pulmonary CD8 T cells. Functional IFN-gamma(+) CD8 responses were slightly higher in the FI-bRSV vaccinated animals. Furthermore, the existence of strong IFN-gamma(+) CD8 responses in FI-bRSV vaccinated animals after challenge suggests (i) that these IFN-gamma(+) responses in FI-bRSV immunized animals do not protect against immunopathology, and (ii) that Th-2 biased responses during bRSV challenge after vaccination with FI-bRSV have a limited impact on the CD8 responses in the bronchoalveolar lavage fluid. Thus, several response patterns (Th-1/Th-2) seem to co-exist during bRSV infection. (c) 2005 Elsevier Ltd. All rights reserved.