MerF is a mercury transport protein: different structures but a common mechanism for mercuric ion transporters?
MerF is a mercury transport protein: different structures but a common mechanism for mercuric ion transporters?
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DOI：
10.1016/s0014-5793(00)01430-7
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发表时间：
2000-04-21
期刊：
影响因子：
3.5
通讯作者：
Brown, NL
中科院分区：
文献类型：
--
作者：
Wilson, JR;Leang, C;Brown, NL
我们已经证明，来自质粒pMER327/419的merF基因编码一个8.7 kDa的汞转运蛋白，通过测定merF在汞还原酶存在下表达时的体内汞挥发，Ne已经证实Tn21的MerC也是一个汞离子转运蛋白。我们已经能够检测到质周蛋白MerP仅与MerT转运体相互作用，而不与MerF或MerC相互作用。亲水性分析预测MerT、MerC和MerF的模型分别具有3个、4个和2个跨膜区域，在所有三种情况下，预测一对半胱氨酸残基在内膜内，另一对半胱氨酸残基在细胞质表面，第二个螺旋含有脯氨酸和至少一个带电荷的残基。汞离子在这些转运体中的转运机制可能相似，尽管它们在膜中的结构不同。(C) 2000年欧洲生化学会联合会。
Mercury resistance determinants are widespread in cram-negative bacteria, but vary in the number and identity of genes present, We have shown that the merF gene from plasmid pMER327/419 encodes a 8.7 kDa mercury transport protein, by determining in vivo mercury volatilisation when MerF is expressed in the presence of mercuric reductase, Ne have confirmed that MerC of Tn21 is also a mercuric ion transporter. We have been able to detect interaction of the periplasmic protein MerP only with the MerT transporter, and not with MerF or MerC. Hydropathy analysis led to the prediction of models for MerT, MerC and MerF having three, four and two transmembrane regions respectively, In all three cases one pair of cysteine residues is predicted to be within the inner membrane with a second pair of cysteine residues on the cytoplasmic face, and the second helix contains a proline and at least one charged residue. The mechanisms of mercuric ion transport may be similar in these transporters even though their structures in the membrane differ. (C) 2000 Federation of European Biochemical Societies.