Immunofluorescent and immunoelectron microscopic localization of protein antigens in red cells infected with the human malaria Plasmodium falciparum.
Immunofluorescent and immunoelectron microscopic localization of protein antigens in red cells infected with the human malaria Plasmodium falciparum.
复制标题
感染人类疟疾恶性疟原虫的红细胞中蛋白质抗原的免疫荧光和免疫电子显微镜定位。
DOI：
10.1080/00034983.1988.11812289
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发表时间：
1988
影响因子：
--
通讯作者：
delaVega,P
中科院分区：
文献类型：
--
作者：
Sherman,IW;Greenan,JR;delaVega,P
Erythrocytes infected with the human malariaPlasmodium falciparumproduce elevations of the surface membrane of the red cell called knobs. Through the use of transmission electron microscopy and a post-embedding protein A-immunogold technique, it was possible to show changes in the distribution of band 3, glycophorin A and spectrin in the region of the knob. These proteins appeared to be aggregated or condensed in the area of the knob, whereas the remainder of the red cell surface showed no such dense clusters; haemoglobin and the histidine-rich protein ofP. lophuraecould not be localized to the knobby protuberances. It was not possible to detect any changes in protein distribution using the light microscope and indirect immunofluorescence.
