The major pathways of carbohydrate metabolism in the ectomycorrhizal basidiomycete Laccaria bicolor S238N
The major pathways of carbohydrate metabolism in the ectomycorrhizal basidiomycete Laccaria bicolor S238N
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DOI：
10.1111/j.1469-8137.2008.02581.x
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发表时间：
2008-01-01
期刊：
影响因子：
9.4
通讯作者：
Martin, F.
中科院分区：
文献类型：
--
作者：
Deveau, A.;Kohler, A.;Martin, F.
外生菌根真菌的主要碳水化合物代谢及其转录调控从来没有在基因组规模的特点，虽然它在共生的功能发挥了重要作用。本研究利用外生菌根真菌双色乳菇（Laccaria bicolor）S238 N-H82的全基因组序列，对糖酵解、磷酸戊糖途径、三羧酸循环、海藻糖和甘露醇代谢等相关基因和基因家族进行了注释，构建了一个完整的碳水化合物初级代谢途径基因库。利用全基因组表达寡核苷酸芯片和定量聚合酶链反应研究了这些途径在自由生活的菌丝体、外生菌根和子实体中的转录调控。外生菌根和子实体发育诱导糖酵解、甘露醇和海藻糖代谢的一组限制性转录本的调节。
The primary carbohydrate metabolism of an ectomycorrhizal fungus and its transcriptional regulation has never been characterized at the genome scale although it plays a fundamental role in the functioning of the symbiosis. In this study, the genome sequence of the ectomycorrhizal basidiomycete Laccaria bicolor S238N-H82 was explored to construct a comprehensive genome-wide inventory of pathways involved in primary carbohydrate metabolism.Several genes and gene families were annotated, including those of the glycolysis, pentose phosphate pathway, tricarboxylic acid cycle, and trehalose and mannitol metabolism. The transcriptional regulation of these pathways was studied using whole-genome expression oligoarrays and quantitative polymerase chain reaction in free-living mycelium, ectomycorrhizas and fruiting bodies.Pathways of carbohydrate biosynthesis and catabolism are identical in L. bicolor compared with other sequenced saprotrophic basidiomycetes.Ectomycorrhiza and fruiting body development induced the regulation of a restricted set of transcripts of the glycolytic, mannitol and trehalose metabolisms.