INHIBITION AND ACTIVATION OF BACTERIAL LUCIFERASE SYNTHESIS
INHIBITION AND ACTIVATION OF BACTERIAL LUCIFERASE SYNTHESIS
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DOI：
10.1128/jb.109.3.1101-1105.1972
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发表时间：
1972-01-01
影响因子：
3.2
通讯作者：
EBERHARD, A
中科院分区：
文献类型：
--
作者：
EBERHARD, A
当将生物发光细菌接种到新鲜培养基中时，荧光素酶的合成被抑制，但在细胞在培养基中生长一段时间后，荧光素酶的合成被诱导。在基本培养基中，细菌将导致酶诱导的活化剂释放到培养基中。完全培养基含有可透析且相当稳定的抑制剂，其导致荧光素酶的抑制。细菌从培养基中去除抑制剂，并产生激活剂，从而允许酶的合成。两种未鉴定的非发光细菌菌株无法去除抑制剂。两种不同发光菌株，费氏发光杆菌（Photobacterium fischeri）和P. fischeristrain MAV产生对其它菌株的细胞无效的特异性激活剂。这两种活化剂在热稳定性方面不同，但都是小分子。活化剂可以根据其对抗抑制剂的能力进行测定。抑制剂和激活剂的识别可以使生物发光系统与细胞的其他代谢过程联系起来。
Luciferase synthesis is repressed when bioluminescent bacteria are inoculated into fresh medium but is induced after the cells have grown in the medium for some time. In minimal medium, an activator which leads to induction of the enzyme is released into the medium by the bacteria. Complete medium contains a dialyzable and quite stable inhibitor which leads to repression of luciferase. The bacteria remove the inhibitor from the medium and also produce activator, thus allowing synthesis of the enzyme. Two unidentified nonluminescent strains of bacteria were unable to remove the inhibitor. Two different bioluminescent strains,Photobacterium fischeriandP. fischeristrain MAV, produce specific activators that are ineffective with cells of the other strain. The two activators are different with respect to heat stability, but both are small molecules. The activators can be assayed on the basis of their ability to counteract the inhibitor. Identification of the inhibitor and the activators may allow the bioluminescent system to be linked to other metabolic processes of the cells.