Immunomapping of desmosomal and nondesmosomal adhesion molecules in healthy canine footpad, haired skin and buccal mucosal epithelia: comparison with canine pemphigus foliaceus serum immunoglobulin G staining patterns
Immunomapping of desmosomal and nondesmosomal adhesion molecules in healthy canine footpad, haired skin and buccal mucosal epithelia: comparison with canine pemphigus foliaceus serum immunoglobulin G staining patterns
复制标题
DOI：
10.1111/j.1365-3164.2010.00924.x
复制
发表时间：
2011-04-01
影响因子：
1.4
通讯作者：
Olivry, Thierry
中科院分区：
文献类型：
--
作者：
Bizikova, Petra;Linder, Keith E.;Olivry, Thierry
叶状天疱疮（PF）是最常见的犬自身免疫性皮肤病。与人类PF （hPF）相反，在犬疾病中，粘粒蛋白-1是一种次要的自身抗原。犬PF （cPF）的主要自身抗原(s)仍然未知，这限制了进行病变形成机制研究和开发新的诊断和治疗策略的能力。采用hPF、寻常型天疱疮血清和特异性抗体检测健康犬足垫、毛皮肤和颊粘膜上皮组织中选定的桥粒蛋白（桥粒蛋白-1、桥粒蛋白-3、桥粒蛋白-1、桥粒蛋白-3、桥粒蛋白-1、桥粒蛋白-1、桥粒蛋白-1）和非桥粒粘附蛋白（E-cadherin、claudin-1、occludin）的免疫荧光模式。并与66份cPF血清间接免疫荧光染色结果进行比较。大多数cPF血清（58 / 66,88%）沿犬足垫棘层和颗粒层角化细胞边缘呈阳性染色。一种血清含有仅与颗粒角质形成细胞结合的自身抗体。在66份cPF血清中，基底层的细胞间同步荧光仅限于7份（11%）。66份cPF血清中只有12份（18%）显示口腔黏膜IF染色阳性。本研究证实了cPF免疫球蛋白G自身抗体的免疫学异质性。此外，主要的间接免疫荧光染色模式和大多数cPF血清不能标记口腔黏膜的情况与桥黏菌素-1密切匹配。这些观察结果为进一步研究desmocolin -1作为潜在的主要cPF自身抗原提供了依据。
Pemphigus foliaceus (PF) is the most common canine autoimmune skin disease. In contrast to human PF (hPF), desmoglein-1 is a minor autoantigen in the canine disease. The major autoantigen(s) of canine PF (cPF) remain(s) unknown, which limits the ability to perform mechanistic studies of lesion formation and the development of novel diagnostic and therapeutic strategies for this disease. The immunofluorescence patterns of selected desmosomal (desmoglein-1, desmoglein-3, desmocollin-1, desmocollin-3, desmoplakin-1/2, plakoglobin and plakophilin-1) and nondesmosomal adhesion proteins (E-cadherin, claudin-1, zona occludens-1 and occludin) in healthy canine footpad, haired skin and buccal mucosal epithelia were determined using hPF and pemphigus vulgaris sera and specific antibodies. The immunostaining patterns were then compared with that of indirect immunofluorescence staining with 66 cPF sera. Most cPF sera (58 of 66; 88%) exhibited positive staining along keratinocyte margins in the stratum spinosum and stratum granulosum of canine footpad. One serum contained autoantibodies binding solely to stratum granulosum keratinocytes. Concurrent intercellular fluorescence in the stratum basale was limited to seven of 66 cPF sera (11%). Only 12 of 66 cPF sera (18%) also exhibited positive IF staining of the buccal mucosa. This study confirms the immunological heterogeneity of cPF immunoglobulin G autoantibodies. Moreover, the major indirect immunofluorescence staining pattern and the inability of most cPF sera to label the buccal mucosa closely matched that of desmocollin-1. These observations warrant further investigation of desmocollin-1 as a potential major cPF autoantigen.