Cutting Edge: TGF-β1 and IL-15 Induce FOXP3+ γδ Regulatory T Cells in the Presence of Antigen Stimulation
Cutting Edge: TGF-β1 and IL-15 Induce FOXP3+ γδ Regulatory T Cells in the Presence of Antigen Stimulation
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DOI：
10.4049/jimmunol.0901334
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发表时间：
2009-09-15
影响因子：
4.4
通讯作者：
Malkovsky, Miroslav
中科院分区：
文献类型：
--
作者：
Casetti, Rita;Agrati, Chiara;Malkovsky, Miroslav
对α-β调节性T细胞(Tregs)的几个亚群已经进行了深入的描述和研究，但Gamma-Delta T细胞的潜在调节作用在很大程度上仍不清楚。表达γ-TCR的淋巴细胞既参与先天免疫反应，又参与获得性免疫反应，其主要的成人外周血亚群(V-Gamma 9V Delta 52)对微生物制剂和肿瘤具有广泛的反应性。在这项研究中，我们报道了具有调节功能的γ-Delta T淋巴细胞(V-Delta 2 Tregs)在体外被特异性的抗原刺激和细胞因子(转化生长因子-β1和IL-15)诱导。这些细胞表达FOXP3，与α-βTregs类似，能抑制抗CD3/抗CD28刺激的PBMC的增殖。V Delta 2树突状细胞的表型和功能分析将极有可能提高我们对Gamma Delta T细胞在自身免疫性、感染性和肿瘤性疾病发病机制中的作用的理解。免疫学杂志，2009,183：3574-3577。
Several subsets of alpha beta regulatory T cells (Tregs) have been described and studied intensively, but the potential regulatory role of gamma delta T cells remains largely unclear. Lymphocytes expressing gamma delta TCR are involved in both innate and adaptive immune responses, and their major adult human peripheral blood subset (V gamma 9V delta 52) displays a broad reactivity against microbial agents and tumors. In this study we report that gamma delta T lymphocytes with regulatory functions (V delta 2 Tregs) are induced in vitro in the presence of specific Ag stimulation and cytokines (TGF-beta 1 and IL-15). These cells express FOXP3 and, similarly as alpha beta Tregs, suppress the proliferation of anti-CD3/anti-CD28 stimulated-PBMC. Phenotypic and functional analyses of V delta 2 Tregs will very likely improve our understanding about the role of gamma delta T cells in the pathogenesis of autoimmune, infectious, and neoplastic diseases. The Journal of Immunology, 2009, 183: 3574-3577.