Induction of lymphoid leukosis transplant able tumours and the establishment of lymphoblastoid cell lines.
Induction of lymphoid leukosis transplant able tumours and the establishment of lymphoblastoid cell lines.
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诱导淋巴白血病可移植肿瘤并建立淋巴母细胞系。
DOI：
10.1080/03079458008418416
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
D. Ewert
中科院分区：
文献类型：
--
作者：
W. Okazaki;R. Witter;C. Romero;K. Nazerian;Jagdev M. Sharma;Aly M. Fadly;D. Ewert
分别由亚群A、B、C和D淋巴样白血病病毒RAV-1、RAV-2、RAV-49和RAV-50诱导的原发性淋巴样肿瘤已被开发为可移植的“B细胞”淋巴样肿瘤，并分别命名为LSCT-RP 5、LSCT-RP 6、LSCT-RP 7和LSCT-RP 8。毫无疑问，这些确实是移植物，因为所有这些都在很短的时间内产生肿瘤和接种鸡的死亡（死亡的平均天数=5-10天），染色体分析表明，当在异性鸡中传代时，肿瘤细胞的性别保持不变。我们已经从RAV-2可移植肿瘤（LSCT-RP 6）建立了2个体外淋巴母细胞系（LSCC-RP 9和LSCC-RP 12），但是在用RAV-1可移植肿瘤（LSCT-RP 5）进行多次尝试后没有成功。LSCC-RP 9和LSCC-RP 12淋巴母细胞样细胞系生长迅速，并产生大量病毒。这些可移植的肿瘤和淋巴母细胞系应提供有价值的材料，研究病毒宿主和肿瘤细胞宿主的相互关系。
Primary lymphoid tumours induced by the subgroup A, B, C and D lymphoid leukosis viruses RAV-1, RAV-2, RAV-49 and RAV-50, respectively, have been developed as transplantable "B-cell" lymphoid tumours and designated LSCT-RP5, LSCT-RP6, LSCT-RP7 and LSCT-RP8, respectively. There seems little doubt that these are indeed transplants because all produce tumours and death of the inoculated chicks in a very short time (median days to death =5-10 days), and chromosome analyses reveal that the sex of the tumour cells remains unchanged when passaged in chicks of the opposite sex. We have established 2 in vitro lymphoblastoid lines (LSCC-RP9 and LSCC-RP12) from the RAV-2 transplantable tumour (LSCT-RP6), but were unsuccessful after numerous attempts with RAV-1 transplantable tumours (LSCT-RP5). The LSCC-RP9 and LSCC-RP12 lymphoblastoid cell lines grow rapidly and produce large amounts of virus. These transplantable tumours and lymphoblastoid cell lines should provide valuable materials for studies on virus-host and tumour cell-host interrelationships.