Genomic structure of mouse PIR-A6, an activating member of the paired immunoglobulin-like receptor gene family.
Genomic structure of mouse PIR-A6, an activating member of the paired immunoglobulin-like receptor gene family.
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小鼠 PIR-A6 的基因组结构，PIR-A6 是配对免疫球蛋白样受体基因家族的激活成员。
DOI：
10.1034/j.1399-0039.2003.00042.x
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Kubagawa,H
中科院分区：
文献类型：
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作者：
Tun,T;Kubagawa,Y;Dennis,G;Burrows,PD;Cooper,MD;Kubagawa,H
从基因组文库中分离成对IG样受体家族激活成员之一Pira 6的基因并测序。Pira 6基因的9个外显子中的第一个外显子编码5′非翻译区，翻译起始位点和大约一半的信号序列。 第二个外显子编码信号序列的其余部分，外显子3-8各编码单个IG样胞外结构域，外显子9编码跨膜区、胞质尾区和3′ UTR，具有4个多聚腺苷酸化信号和6个mRNA不稳定序列。PIR-A6的可溶形式可以通过选择性剪接产生。Pira 6基因的外显子序列占总序列的42%，Pirb基因的外显子序列占总序列的34%，因此Pira 6和Pirbas基因的内含子序列相对较短。Pira 6和Pirb之间从ATG起始位点上游约2kb到内含子8的起始处存在广泛的序列同源性。 ThePirgenes似乎分布在3个区域的近端的染色体7的基础上，目前的数据和分析目前可用的小鼠基因组序列数据库。其中一个区域含有一个与Pira 6几乎相同的Pirgene，另外两个区域含有多个转录方向相反的Pirgene。在Pira 6转录起始位点上游（位于启动子共有序列基序内）鉴定了造血特异性和普遍存在的转录因子的潜在结合位点。这些结果为在造血过程中观察到的PIR-A和PIR-B表达的协调调节提供了重要线索。
The gene for one of the activating members of the paired Ig‐like receptor family,Pira6, was isolated from a genomic library and sequenced. The first of 9 exons in the ∼8.2 kbPira6gene encodes the 5′ untranslated region, the translation initiation site, and approximately half of the signal sequence. The second exon encodes the rest of the signal sequence, exons 3–8 each encode a single Ig‐like extracellular domain, and exon 9 encodes the transmembrane region, cytoplasmic tail and 3′ UTR with four polyadenylation signals and six mRNA instability sequences. A soluble form of PIR‐A6 may be generated by alternative splicing. The exonic sequences account for ∼42% of thePira6gene and ∼34% for the single inhibitoryPirbgene, thus definingPiraandPirbas genes with relatively short intronic sequences. Extensive sequence homology was found betweenPira6andPirbfrom ∼2 kb upstream of the ATG initiation site to the beginning of intron 8. ThePirgenes appear to be distributed in three regions of the proximal end of chromosome 7 based on the present data and an analysis of currently available mouse genomic sequence databases. One region contains a singlePirgene which is almost identical toPira6, and the other two contain multiplePirgenes in opposite transcriptional orientations. Potential binding sites for hemopoiesis‐specific and ubiquitous transcription factors were identified upstream of thePira6transcription start sites that reside within the initiator consensus sequence motif. These results provide important clues to the coordinate regulation observed for PIR‐A and PIR‐B expression during hematopoiesis.