Platelet-rich plasma-derived fibrin clot formation stimulates collagen synthesis in periodontal ligament and osteoblastic cells in vitro
Platelet-rich plasma-derived fibrin clot formation stimulates collagen synthesis in periodontal ligament and osteoblastic cells in vitro
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DOI：
10.1902/jop.2003.74.6.858
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发表时间：
2003-06-01
影响因子：
4.3
通讯作者：
Yoshie, H
中科院分区：
文献类型：
--
作者：
Kawase, T;Okuda, K;Yoshie, H
背景资料：富血小板血浆（PRP）含有多种生长因子，包括高水平的血小板衍生生长因子（PDGF）和转化生长因子-β（TGF-β）。我们已经证明PRP的功能类似于TGF-β，以细胞类型特异性的方式调节细胞增殖。此外，PRP在几种（但不是所有）测试的细胞培养物中形成凝胶样物质。本研究旨在探讨PRP对牙周膜（PDL）和成骨细胞MG 63细胞分泌细胞外基质的作用。用PRP（0.5%至2%）处理的细胞进行I型胶原和纤维蛋白免疫细胞化学染色，并目视评价培养基的粘度。免疫斑点法检测PRP中纤维蛋白原的表达，酶联免疫吸附法检测细胞内内源性凝血酶的表达。
Background: Platelet-rich plasma (PRP) contains several growth factors, including platelet-derived growth factor (PDGF) and transforming growth factor-beta (TGF-beta), at high levels. We have demonstrated the PRP functions like TGF-beta to modulate cell proliferation in a cell-type specific manner. In addition, PRP forms gel-like materials in several, but not all, cell cultures tested. This study was designed to investigate PRP's action on extracellular matrix production in periodontal ligament (PDL) and osteoblastic MG63 cell cultures.Methods: PRP was prepared from the plasma obtained from autologous blood of healthy volunteers and stored at -20degreesC until used. Cells treated with PRP (0.5% to 2%) were immunocytochemically stained for type I collagen and fibrin and the viscosity of the culture media was visually evaluated. Fibrinogen in PRP was detected by immunodot-blotting while endogenous thrombin expression in cells was detected by a modified enzyme-linked immunosorbent assay.Results: Gel-like material rapidly (