Enhanced kefiran production by mixed culture of Lactobacillus kefiranofaciens and Saccharomyces cerevisiae
Enhanced kefiran production by mixed culture of Lactobacillus kefiranofaciens and Saccharomyces cerevisiae
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DOI：
10.1016/s0168-1656(02)00228-6
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发表时间：
2003-01-09
影响因子：
4.1
通讯作者：
Shioya, S
中科院分区：
文献类型：
--
作者：
Cheirsilp, B;Shimizu, H;Shioya, S
采用乳酸菌和酿酒酵母混合培养的方法，研究了乳酸菌和酿酒酵母混合培养对乳酸菌生长和发酵的影响。与纯培养物相比，Kefiranofaciens显著增加。在厌氧条件下，混合培养的发酵速率为36 mg·l（-1）·h（-1），高于纯培养的发酵速率（24 mg·l（-1）·h（-1））。在好氧条件下，两株菌之间的相互作用更强，发酵速率（44 mg l（-1）h（-1））比厌氧条件下高。通过在补料分批混合培养中加入新鲜培养基进一步增强开非仑生产。在补料分批混合培养中，在87 h时达到5.41 g l（-1）的最终开非仑浓度，从而达到62 mg l（-1）h（-1）的最高生产率。通过模拟实验研究了乳酸菌纯培养物中乳酸的减少情况，以评估与S.啤酒。观察到混合培养物中的细胞生长和开非仑生产速率略高于通过模拟从纯培养物中预期的细胞生长和开非仑生产速率。这些结果表明，L。kefiranofaciens和S.酿酒酵母不仅可以通过消耗降低乳酸浓度，而且还可以刺激乳酸菌的细胞生长和开菲尔聚糖的产生。开菲尔诺菌(C)2002 Elsevier Science B. V.保留所有权利。
In a batch mixed culture of Lactobacillus kefiranofaciens and Saccharomyces cerevisiae, which could assimilate lactic acid, cell growth and kefiran production rates of L. kefiranofaciens significantly increased, compared with those in pure cultures. The kefiran production rate was 36 mg l(-1) h(-1) in the mixed culture under the anaerobic condition, which was greater than that in the pure culture (24 mg l(-1) h(-1)). Under the aerobic condition, a more intensive interaction between these two strains was observed and higher kefiran production rate (44 mg l(-1) h(-1)) was obtained compared with that under the anaerobic condition. Kefiran production was further enhanced by an addition of fresh medium in the fed-batch mixed culture. In the fed-batch mixed culture, a final kefiran concentration of 5.41 g l(-1) was achieved at 87 h, thereby attaining the highest productivity at 62 mg l(-1) h(-1). Simulation study considered the reduction of lactic acid in pure culture was performed to estimate the additional effect of coculture with S. cerevisiae. Slightly higher cell growth and kefiran production rates in the mixed culture than those expected from pure culture by simulation were observed. These results suggest that coculture of L. kefiranofaciens and S. cerevisiae not only reduces the lactic acid concentration by consumption but also stimulates cell growth and kefiran production of L. kefiranofaciens. (C) 2002 Elsevier Science B.V. All rights reserved.