Identifying a Major QTL Associated with Salinity Tolerance in Nile Tilapia Using QTL-Seq
Identifying a Major QTL Associated with Salinity Tolerance in Nile Tilapia Using QTL-Seq
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使用 QTL-Seq 鉴定与尼罗罗非鱼耐盐性相关的主要 QTL
DOI：
10.1007/s10126-017-9790-4
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发表时间：
2018-02-01
影响因子：
3
通讯作者：
Xia, Jun Hong
中科院分区：
文献类型：
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作者：
Gu, Xiao Hui;Jiang, Dan Li;Xia, Jun Hong
选择具有高耐盐性的新品系允许在微咸水区经济可行地生产罗非鱼。定位罗非鱼耐盐性状的QTL和筛选与耐盐性状相关的标记是利用分子标记辅助选择技术改良罗非鱼耐盐性的第一步。利用QTL-seq策略和连锁分析方法，首次在尼罗罗非鱼中定位到2个耐盐性状的显著QTL区间chrLG 4和chrLG 18。微卫星和SNP标记的精细定位表明，一个主效QTL区域位于chrLG 18的23.0 Mb处，可解释79%的表型变异，LOD值为95。表达分析表明至少10个基因（例如，LACTB 2、KINH、NC 0A 2、DIP 2C、LARP 4 B、PEX 5 R和KCNJ 9）在10、15或20 ppt攻击下在肠、脑或鳃中显著差异表达。本研究结果表明，QTL-seq可以有效地用于鱼类耐盐性状的QTL定位。该主效QTL为进一步研究罗非鱼耐盐性的遗传机制提供了一个有希望的位点。
Selection of new lines with high salinity tolerance allows for economically feasible production of tilapias in brackish water areas. Mapping QTLs and identifying the markers linked to salinity-tolerant traits are the first steps in the improvement of the tolerance in tilapia through marker-assisted selection techniques. By using QTL-seq strategy and linkage-based analysis, two significant QTL intervals (chrLG4 and chrLG18) on salinity-tolerant traits were firstly identified in the Nile tilapia. Fine mapping with microsatellite and SNP markers suggested a major QTL region that located at 23.0 Mb of chrLG18 and explained 79% of phenotypic variation with a LOD value of 95. Expression analysis indicated that at least 10 genes (e.g., LACTB2, KINH, NCOA2, DIP2C, LARP4B, PEX5R, and KCNJ9) near or within the QTL interval were significantly differentially expressed in intestines, brains, or gills under 10, 15, or 20 ppt challenges. Our findings suggest that QTL-seq can be effectively utilized in QTL mapping of salinity-tolerant traits in fish. The identified major QTL is a promising locus to improve our knowledge on the genetic mechanism of salinity tolerance in tilapia.