Electrochemical and Spectroscopic Studies on the Interaction of Malachite Green with DNA and Its Application
Electrochemical and Spectroscopic Studies on the Interaction of Malachite Green with DNA and Its Application
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DOI：
10.1002/elan.200503438
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发表时间：
2006-03
期刊：
影响因子：
3
通讯作者：
Xuan Hu;K. Jiao;Wei Sun;J. You
中科院分区：
文献类型：
--
作者：
Xuan Hu;K. Jiao;Wei Sun;J. You
用电化学和分光光度法研究了在pH 7.0Britton-Robinson(B-R)缓冲溶液中孔雀石绿(MG)与双链DNA(DsDNA)的相互作用。在扫描速度为0.20V/−的循环伏安图上，MG在+1.5V(vs.SCE)的电位扫描范围内，在0.547 V和0.833 V有两个氧化峰，在0.362 V有一个还原峰，加入dsDNA后，0.547 V的氧化峰电流明显减小。计算并比较了dsDNA存在和无dsDNA存在时的电荷转移系数(α)、表面反应速率常数(Ks)和扩散系数(D)等电化学参数。结果表明，加入dsDNA后MG的这些参数发生了很大的变化，表明形成了一种电化学活性络合物。从电化学和UV-Vis光谱等方面详细讨论了MG与dsDNA的相互作用机理。利用加入dsDNA后MG峰电流的降低，进一步用于差示脉冲伏安法(DPV)测定dsDNA。双链DNA的线性范围为10.0~100.0μg/m L，线性回归方程为：ΔIP(μA)=0.065+0.0096 C(μg/m L)，检出限为6.0μg/m L(3σ)。考察了共存物质的影响，并对人工样品进行了测定，结果令人满意。
The interaction of malachite green (MG) with double-stranded DNA (dsDNA) in pH 7.0 Britton–Robinson (B–R) buffer solution was investigated by electrochemical and spectrophotometric methods. Within the potential scan range of −1.0 to +1.5 V (vs. SCE), MG has two oxidative peaks at 0.547 V and 0.833 V and one reductive peak at 0.362 V on cyclic voltammogram at the scan rate of 0.20 V/s. After the addition of dsDNA into the MG solution, the oxidative peak current at 0.547 V decreases obviously. The electrochemical parameters, such as the charge transfer coefficient (α), the surface reaction rate constant (ks) and the diffusion coefficient (D), were calculated and compared between in the absence and presence of dsDNA. The results show that these parameters of MG after adding dsDNA have greatly changed, which indicates that an electrochemical active complex was formed. The interaction mechanisms of MG with dsDNA are discussed in some details from the electrochemistry and UV-vis spectrophotometry. The reduction of the peak current of MG after adding dsDNA was further used for the quantification of dsDNA by differential pulse voltammetry (DPV). The linear range for dsDNA is in the range of 10.0–100.0 μg/mL with the linear regression equation as Δip (μA)=0.065+0.0096 C (μg/mL) and the detection limit of 6.0 μg/mL (3σ). The influences of coexisting substances were investigated and artificial samples were determined with satisfactory results.