Transcriptomic analysis of genes involved in the biosynthesis, recycling and degradation of L-ascorbic acid in pepper fruits (Capsicum annuum L.)
Transcriptomic analysis of genes involved in the biosynthesis, recycling and degradation of L-ascorbic acid in pepper fruits (Capsicum annuum L.)
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DOI：
10.1016/j.plantsci.2013.02.007
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发表时间：
2013-06-01
期刊：
影响因子：
5.2
通讯作者：
Zacarias, Lorenzo
中科院分区：
文献类型：
--
作者：
Alos, Enriqueta;Rodrigo, Maria J.;Zacarias, Lorenzo
甜椒（Capsicum annuum L.）是公认的抗坏血酸（阿萨）含量最高的蔬菜。然而，参与阿萨的生物合成、循环和降解的代谢途径及其对阿萨浓度的相对贡献尚未确定。在本工作中，选择参与阿萨的生物合成，降解和回收途径的基因的表达水平进行了分析，在辣椒果实的发育和成熟。巴勒莫和四个品种（Lipari，C-116，Surrentino和Italverde）的成熟果实中的不同阿萨浓度。辣椒果实中生物合成基因的表达与阿萨浓度呈负相关，表明辣椒果实中存在反馈机制调节阿萨的稳态。有趣的是，抗坏血酸氧化酶的mRNA水平的分析，参与了阿萨的降解，表明这种酶在调节阿萨池在果实发育和成熟过程中起着至关重要的作用。(C)2013爱思唯尔爱尔兰有限公司版权所有。
Sweet pepper (Capsicum annuum L) is widely recognized among the vegetables with high content of ascorbic acid (AsA). However, the metabolic pathways involved in the biosynthesis, recycling and degradation of AsA and their relative contribution to the concentration of AsA have not been established yet. In the present work, the expression levels of selected genes involved in the AsA biosynthesis, degradation and recycling pathways were analyzed during development and ripening of pepper fruit cv. Palermo and in mature fruit of four cultivars (Lipari, C-116, Surrentino and Italverde) with different AsA concentrations. An inverse correlation was found between the expression of the biosynthetic genes and AsA concentrations, which could indicate that a feedback mechanism regulates AsA homeostasis in pepper fruits. Interestingly, analysis of mRNA levels of ascorbate oxidase, involved in the degradation of AsA, suggests that this enzyme plays a critical role in the regulation of the AsA pool during fruit development and ripening. (C) 2013 Elsevier Ireland Ltd. All rights reserved.