Estrogen and androgen regulate actin-remodeling and endocytosis-related genes during rat spermiation
Estrogen and androgen regulate actin-remodeling and endocytosis-related genes during rat spermiation
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DOI：
10.1016/j.mce.2014.12.029
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发表时间：
2015-03-15
影响因子：
4.1
通讯作者：
Balasinor, N. H.
中科院分区：
文献类型：
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作者：
Kumar, Anita;Dumasia, Kushaan;Balasinor, N. H.
精子作用，即精子的释放过程，对男性的生育和繁殖至关重要。形态学上，它的特征是去除非典型粘附连接（称为外质特化），形成瞬时内吞装置（称为管小球复合物），需要细胞骨架重塑和内吞作用所需的蛋白质募集。早些时候，雌激素给成年雄性大鼠被认为是由于破坏管小球复合物而导致精子失败。这伴随着睾丸内睾丸激素水平的降低和睾丸内雌激素水平的增加，以及参与细胞骨架重塑（Arpc1b， Evl和Capg）和内吞作用（Picalm， Eea1和Stx5 α）的基因的解除。在本研究中，我们旨在通过用雌激素、雄激素或雌激素受体的激动剂和拮抗剂处理的精管培养系统，了解雌激素和雄激素在独立调节这些基因中的作用。我们发现Arpc1b、Evl和Picalm转录本对雌激素有反应，而Picalm、Eea1和Stx5a转录本对雄激素有反应。我们还发现雌激素对Arpc1b和Evl的调节是通过雌激素受体β介导的，Picalm的调节是通过雌激素受体a和β介导的。这些蛋白在小管球复合体或其附近的定位表明，ARPC1B、EVL、PICALM、EEA1和STX5A似乎参与了精子形成。因此，雌激素和雄激素调节了精管中可能在精子受精中起作用的特定基因。2014爱思唯尔爱尔兰有限公司版权所有。
Spermiation, the sperm release process, is imperative to male fertility and reproduction. Morphologically, it is characterized by removal of atypical adherens junctions called ectoplasmic specializations, and formation of transient endocytic devices called tubulobulbar complexes requiring cytoskeleton remodeling and recruitment of proteins needed for endocytosis. Earlier, estrogen administration to adult male rats was seen to cause spermiation failure due to disruption of tubulobulbar complexes. This was accompanied by reduction in intratesticular testosterone levels and increase in intratesticular estrogen along with deregulation of genes involved in cytoskeleton remodeling (Arpc1b, Evl and Capg) and endocytosis (Picalm, Eea1 and Stx5 alpha). In the present study, we aim to understand the role of estrogen and androgen in regulating these genes independently using seminiferous tubule culture system treated with estrogen, androgen or agonists and antagonists of estrogen receptors. We find that transcripts of Arpc1b, Evl and Picalm are responsive to estrogen while those of Picalm, Eea1 and Stx5a are responsive to androgen. We also find that the estrogen regulation of Arpc1b and Evl is mediated through estrogen receptor beta and that of Picalm occurs through estrogen receptors a and beta. Localization of these proteins at or in the vicinity of tubulobulbar complexes reveals that ARPC1B, EVL, PICALM, EEA1 and STX5A seem to be involved in spermiation. Thus, estrogen and androgen regulate specific genes in seminiferous tubules that could play a role in spermiation. (C) 2014 Elsevier Ireland Ltd. All rights reserved.