Complete nucleotide sequence of avian paramyxovirus type 6 isolated from ducks
Complete nucleotide sequence of avian paramyxovirus type 6 isolated from ducks
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DOI：
10.1099/0022-1317-82-9-2157
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发表时间：
2001-09-01
影响因子：
3.8
通讯作者：
Shieh, HK
中科院分区：
文献类型：
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作者：
Chang, PC;Hsieh, ML;Shieh, HK
禽副粘病毒（APMV）有九种血清型。只有新城疫病毒1型（APMV1）的基因组已被完全测序。在这项研究中，报告了从鸭中分离的APMV-6血清型的完整核苷酸序列。16236 nt基因组编码8个蛋白，分别为核衣壳蛋白（NP）、磷蛋白(P)、V蛋白、基质蛋白(M)、融合蛋白(F)、小疏水蛋白（SH）、血凝素-神经氨酸酶（HN）蛋白和大蛋白(L)，其两侧分别有一个55 nt的先导序列和一个54 nt的尾链序列。序列比较表明，APMV-6蛋白序列与APMV-1 （NDV）和-2蛋白序列的亲缘关系最为密切，序列同源性在22% ~ 44%之间。然而，APMV-6含有一个可能编码SH蛋白的基因，该基因在APMV-1中不存在，但在风疹病毒、类人猿病毒5型和腮腺炎病毒中存在。APMV-6中SH基因的存在可能提供了APMV和风疹病毒进化之间的联系。系统发育分析表明，APMV-6、-1、-2（只有F和HN序列可用于分析）和-4（只有HN序列可用于分析）都聚集在一个单一的谱系中，与其他副粘病毒不同。这一结果表明，APMV应该是副粘病毒亚科中的一个新属。
There are nine serotypes of avian paramyxovirus (APMV). Only the genome of APMV type 1 (APMV1), also called Newcastle disease virus (NDV), has been completely sequenced. In this study, the complete nucleotide sequence of an APMV-6 serotype isolated from ducks is reported. The 16236 nt genome encodes eight proteins, nucleocapsid protein (NP), phosphoprotein (P), V protein, matrix protein (M), fusion protein (F), small hydrophobic (SH) protein, haemagglutinin-neuraminidase (HN) protein and large (L) protein, which are flanked by a 55 nt leader sequence and a 54 nt trailer sequence. Sequence comparison reveals that the protein sequences of APMV-6 are most closely related to those of APMV-1 (NDV) and -2, with sequence identities ranging from 22 to 44%. However, APMV-6 contains a gene that might encode the SH protein, which is absent in APMV-1, but present in the rubulaviruses simian virus type 5 and mumps virus. The presence of an SH gene in APMV-6 might provide a link between the evolution of APMV and rubulaviruses. Phylogenetic analysis demonstrates that APMV-6, -1, -2 (only the F and HN sequences were available for analysis) and -4 (only the HN sequences were available for analysis) all cluster into a single lineage that is distinct from other paramyxoviruses. This result suggests that APMV should constitute a new genus within the subfamily Paramyxovirinae.