Multistep DNA-Templated Synthesis Using a Universal Template
Multistep DNA-Templated Synthesis Using a Universal Template
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使用通用模板进行多步 DNA 模板合成
DOI：
10.1021/ja409936r
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发表时间：
2013-11-27
影响因子：
15
通讯作者：
Li, Xiaoyu
中科院分区：
文献类型：
--
作者：
Li, Yizhou;Zhao, Peng;Li, Xiaoyu
我们报告了一种DNA模板化的合成方法，该方法允许用单个DNA模板构建整个DNA编码文库。利用脱氧次黄苷的无差别碱基配对特性，我们设计了一种“通用模板”，能够指导具有不同序列的多种反应物DNA的化学反应。结合其他设计特征，包括可光裂解的接头和反应物DNA的直接编码，我们证明了通用模板在文库合成、靶选择和命中解码中的能力。我们的方法可以普遍和直接地应用于制备各种化学上不同的DNA编码的文库。
We report a DNA-templated synthesis method that allows construction of the entire DNA-encoded library with a single DNA template. Taking advantage of deoxyinosine's indiscriminate base-pairing property, we designed a "universal template" that is capable of directing chemical reactions with multiple reactant DNAs with different sequences. In combination with other design features including photocleavable linkers and direct encoding by the reactant DNA, we demonstrated the capabilities of the universal template in library synthesis, target selection, and hit decoding. Our method can be generally and straightforwardly applied to prepare a variety of chemically diverse DNA-encoded libraries.