Differentiated analysis of orthodontic tooth movement in rats with an improved rat model and three-dimensional imaging
Differentiated analysis of orthodontic tooth movement in rats with an improved rat model and three-dimensional imaging
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DOI：
10.1016/j.aanat.2013.08.003
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发表时间：
2013-01-01
影响因子：
2.2
通讯作者：
Roemer, Piero
中科院分区：
文献类型：
--
作者：
Kirschneck, Christian;Proff, Peter;Roemer, Piero
目前用于正畸牙齿运动分析的大鼠模型往往缺乏差异化、可靠和精确的测量系统，使研究人员能够分别研究牙齿倾斜、身体平移和牙根扭矩对整体位移的个体贡献。许多先前提出的模型有严重的局限性，例如对正畸力对大鼠门牙的影响的相当不准确的分析。因此，我们开发了一种差异化的测量系统，用于大鼠模型，目的是克服以往研究的局限性。24只雄性Wistar大鼠左上颌第一磨牙和上门牙用镍钛封闭线圈弹簧施加恒定0.25 N的正畸力，持续4周。采用CCD显微镜和锥束计算机断层扫描（CBCT）测量不同类型牙齿的运动程度。两种测量方法均证明连续测量是可靠的，并且引起的明显牙齿移动对动物没有有害影响。运动动力学与已知的生理过程相对应，倾倒和身体运动同样有助于牙齿位移。大鼠的上门牙明显变形，其自然喷发有效地停止。结果表明，我们提出的用于大鼠模型的测量系统解决了以往研究的大部分不足之处。它们是可靠，精确和生理的工具，用于正畸牙齿运动的差异化分析，同时保护动物福利。(C) 2013爱思唯尔有限公司版权所有。
Rat models currently available for analysis of orthodontic tooth movement often lack differentiated, reliable and precise measurement systems allowing researchers to separately investigate-the individual contribution of tooth tipping, body translation and root torque to overall displacement. Many previously proposed models have serious limitations such as the rather inaccurate analysis of the effects of orthodontic forces on rat incisors. We therefore developed a differentiated measurement system that was used within a rat model with the aim of overcoming the limitations of previous studies.The first left upper molar and the upper incisors of 24 male Wistar rats were subjected to a constant orthodontic force of 0.25 N by means of a NiTi closed coil spring for up to four weeks. The extent of the various types of tooth movement was measured optometrically with a CCD microscope camera and cephalometrically by means of cone beam computed tomography (CBCT).Both types of measurement proved to be reliable for consecutive measurements and the significant tooth movement induced had no harmful effects on the animals. Movement kinetics corresponded to known physiological processes and tipping and body movement equally contributed to the tooth displacement. The upper incisors of the rats were significantly deformed and their natural eruption was effectively halted.The results showed that our proposed measurement systems used within a rat model resolved most of the inadequacies of previous studies. They are reliable, precise and physiological tools for the differentiated analysis of orthodontic tooth movement while simultaneously preserving animal welfare. (C) 2013 Elsevier GmbH. All rights reserved.