Ionic Liquid Enables Simple and Rapid Sample Preparation of Human Culturing Cells for Scanning Electron Microscope Analysis
Ionic Liquid Enables Simple and Rapid Sample Preparation of Human Culturing Cells for Scanning Electron Microscope Analysis
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DOI：
10.1002/jemt.20924
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发表时间：
2011-05-01
影响因子：
2.5
通讯作者：
Kuwabata, Susumu
中科院分区：
文献类型：
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作者：
Ishigaki, Yasuhito;Nakamura, Yuka;Kuwabata, Susumu
离子液体是一种即使在室温下也保持熔融状态的盐。它在真空中根本不会蒸发，有助于样品表面导电，以便用扫描电子显微镜 (SEM) 进行观察。在这项研究中，我们首次在扫描电镜中使用离子液体来观察固定的人类培养细胞。使用包裹片和 SEM 设置的细胞培养条件发生变化，以阐明优化的方案。与传统方法制备的样品相比，离子液体处理的样品可以更详细地获得细胞超微结构的 SEM 图像，从而能够观察在分离的细胞之间架起桥梁的微绒毛。此外，与传统的脱水、干燥、电导处理相比，离子液体处理省时、省力。总之，我们得出结论，离子液体是 SEM 样品制备的有用试剂。显微镜。资源。技术。 74:415-420, 2011。(C) 2010 Wiley-Liss, Inc.
Ionic liquid is a kind of salt that stays in a molten state even at room temperature. It does not vaporize at all in vacuum and facilitates electrical conductivity to the sample surfaces for observations with a scanning electron microscope (SEM). In this study, we used an ionic liquid in SEM for the first time to observe fixed human culture cells. The condition for the cell culture using wrapping sheets and SEM settings were varied to elucidate the optimized protocol. Compared to samples prepared by the conventional way, the ionic liquid-treatment of samples gave SEM images of the cellular ultra structures in more detail, enabling observation of microvilli that made bridges between separated cells. In addition, the ionic liquid treatment is less time consuming as well as less laborious compared with the conventional way that includes dehydration, drying, and conductivity treatments. Totally, we concluded the ionic liquid is a useful reagent for SEM sample preparation. Microsc. Res. Tech. 74:415-420, 2011. (C) 2010 Wiley-Liss, Inc.