A Rho-actin signaling pathway shapes cell wall boundaries in Arabidopsis xylem vessels
A Rho-actin signaling pathway shapes cell wall boundaries in Arabidopsis xylem vessels
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DOI：
10.1038/s41467-019-08396-7
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发表时间：
2019-01-28
影响因子：
16.6
通讯作者：
Oda, Yoshihisa
中科院分区：
文献类型：
--
作者：
Sugiyama, Yuki;Nagashima, Yoshinobu;Oda, Yoshihisa
有图案的细胞壁沉积对细胞的形状和功能至关重要。在拟南芥木质部血管中，ROP11 GTPase通过微管分解局部抑制细胞壁沉积，在细胞壁上形成凹孔。在这里，我们发现一个额外的ROP信号通路促进细胞壁在坑边界的生长。ROP域1边界蛋白（Boundary of ROP domain1, BDR1）和壁蛋白（Wallin， WAL）定位于胞穴边界，调控细胞壁生长。WAL与f -肌动蛋白相互作用，促进肌动蛋白在坑边界的组装，而BDR1是ROP效应器。BDR1与WAL相互作用，表明WAL可能通过rop依赖的机制被募集到质膜上。这些结果表明BDR1和WAL介导了形成坑边界的ROP-actin通路。该研究揭示了一种独特的机制，其中两个密切相关的ROP通路相反地调节细胞壁沉积模式，以建立微小但高度特化的细胞壁结构域。
Patterned cell wall deposition is crucial for cell shapes and functions. In Arabidopsis xylem vessels, ROP11 GTPase locally inhibits cell wall deposition through microtubule disassembly, inducing pits in cell walls. Here, we show that an additional ROP signaling pathway promotes cell wall growth at pit boundaries. Two proteins, Boundary of ROP domain1 (BDR1) and Wallin (WAL), localize to pit boundaries and regulate cell wall growth. WAL interacts with F-actin and promotes actin assembly at pit boundaries while BDR1 is a ROP effector. BDR1 interacts with WAL, suggesting that WAL could be recruited to the plasma membrane by a ROP-dependent mechanism. These results demonstrate that BDR1 and WAL mediate a ROP-actin pathway that shapes pit boundaries. The study reveals a distinct machinery in which two closely associated ROP pathways oppositely regulate cell wall deposition patterns for the establishment of tiny but highly specialized cell wall domains.