Herbal Compound "Jiedu Huayu" Reduces Liver Injury in Rats via Regulation of IL-2, TLR4, and PCNA Expression Levels.
Herbal Compound "Jiedu Huayu" Reduces Liver Injury in Rats via Regulation of IL-2, TLR4, and PCNA Expression Levels.
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DOI：
10.1155/2017/9819350
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Long F
中科院分区：
文献类型：
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作者：
Wang M;Shi Q;Zhang R;Qiu H;Mao D;Long F
研究的目的。观察解毒化瘀方对D-氨基半乳糖（D-GalN）和脂多糖诱导的急性肝衰竭（ALF）的预防作用，并探讨其可能的作用机制。材料和方法。用D-半乳糖胺（900 mg/kg）和脂多糖（10 μg/kg）诱导Wistar大鼠ALF。治疗前后测定血清谷丙转氨酶、谷草转氨酶、总胆红素、凝血酶原时间。免疫组织化学染色检测肿瘤细胞核抗原。采用荧光定量逆转录聚合酶链反应（qRT-PCR）和Western blot检测IL-2和TLR 4的表达。结果JDHY治疗显著改善了大鼠ALF模型的肝功能并提高了存活率。使用qRT-PCR和Western印迹分析，我们观察到用JDHY处理ALF大鼠肝细胞后IL-2和TLR 4表达降低。结论我们推测JDHY治疗ALF的治疗潜力是由于其抑制炎症和促进肝细胞再生的调节作用。我们的研究结果有助于验证JDHY在肝病治疗中的传统用途。
Aim of the Study. To investigate the preventative effects of Jiedu Huayu (JDHY) on D-galactosamine (D-GalN) and lipopolysaccharide-induced acute liver failure (ALF) and to evaluate the possible mechanisms of action. Materials and Methods. ALF was induced in Wistar rats by administrating D-GalN (900 mg/kg) and lipopolysaccharide (10 μg/kg). After treatment with JDHY granules, the levels of blood alanine aminotransferase, aspartate aminotransferase, total bilirubin, and prothrombin time were determined. Proliferating cell nuclear antigen was detected by immunohistochemistry staining. The expression of interleukin-2 (IL-2) and toll-like receptor 4 (TLR4) was examined by fluorescence quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot. Results. JDHY treatment dramatically improved liver function and increased survival rates in an ALF model in rats. We observed a decrease in IL-2 and TLR4 expression following treatment with JDHY in liver cells from ALF rats using qRT-PCR and Western blot analysis. Conclusion. We hypothesize that the therapeutic potential of JDHY for treating ALF is due to its modulatory effect on the suppression of inflammation and by promoting hepatocyte regeneration. Our results contribute towards validation of the traditional use of JDHY in the treatment of liver disease.