ANTIBACTERIAL ACTIVITY OF CATIONIC PROTEINS FROM HUMAN GRANULOCYTES
ANTIBACTERIAL ACTIVITY OF CATIONIC PROTEINS FROM HUMAN GRANULOCYTES
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DOI：
10.1172/jci108186
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发表时间：
1975-01-01
影响因子：
15.9
通讯作者：
OLSSON, I
中科院分区：
文献类型：
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作者：
ODEBERG, H;OLSSON, I
人粒细胞含有几种分子量约为25，000的阳离子蛋白质，氨基酸组成几乎相同，并且具有完全的免疫同一性。这些蛋白质具有糜蛋白酶样蛋白酶活性，在中性pH值。阳离子蛋白质的抗菌活性进行了研究。发现对革兰氏阳性（粪链球菌和金黄色葡萄球菌）和革兰氏阴性（大肠杆菌和铜绿假单胞菌）微生物均有杀菌活性。然而，革兰氏阳性菌是最敏感的。最适pH值接近中性，杀微生物活性与离子强度呈反比关系，表明阳离子蛋白质与细菌表面之间存在离子相互作用。阳离子蛋白质的杀微生物作用通常不依赖于相同蛋白质的胰凝乳蛋白酶样活性，因为对几种细菌物种的活性是热稳定的，而胰凝乳蛋白酶样活性是热不稳定的。研究了S.由蛋白A决定的金黄色葡萄球菌似乎不影响对阳离子蛋白的敏感性。本文对E.决定对血清溶解作用敏感性的大肠杆菌，不影响对阳离子蛋白作用的敏感性。目前的研究表明，人类粒细胞的阳离子蛋白代表了一种潜在的杀微生物机制，它不依赖于过氧化氢和髓过氧化物酶。图片
Human granulocytes contain several cationic proteins with a molecular weight of approximately 25,000, almost identical amino acid composition, and complete immunologic identity. These proteins possess a chymotrypsin-like protease activity at a neutral pH. The antibacterial activity of the cationic proteins has been studied. Bactericidal activities are found against both Gram-positive (Streptococcus faecalis and Staphylococcus aureus) and Gram-negative (Escherichia coli and Pseudomonas aeruginosa) organisms. Gram-positive bacteria are, however, the most sensitive. The pH-optimum is near neutrality, and the microbicidal activity shows an inverse relationship to the ionic strength, indicating an ionic interaction between the cationic proteins and the bacterial surface. The microbicidal effect of the cationic proteins is generally independent of the chymotrypsin-like activity of the same proteins since the activity against several bacterial species is heat stable while the chymotrypsin-like activity is heat labile. The surface properties of S. aureus that are determined by protein A do not seem to influence the susceptibility to cationic proteins. The properties of the Gram-negative envelope of E. coli that determine the susceptibility to the lytic action of serum do not influence the sensitivity to the action of cationic proteins. The present study shows that cationic proteins of human granulocytes represent one potential microbicidal mechanism that is independent of hydrogen peroxide and myeloperoxidase.Images