Photoperiod sensitivity of the Arabidopsis circadian clock is tissue-specific.
Photoperiod sensitivity of the Arabidopsis circadian clock is tissue-specific.
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DOI：
10.1080/15592324.2015.1010933
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发表时间：
2015
影响因子：
2.9
通讯作者：
Endo M
中科院分区：
文献类型：
--
作者：
Shimizu H;Araki T;Endo M
最近揭示了拟南芥生物钟的组织特异性功能。与光暗循环下其他组织的时钟相比，脉管系统时钟显示出独特的基因表达谱。然而，尚未确定脉管系统时钟是否也显示出与温度周期（另一个重要的环境线索）相关的独特基因表达模式。在这里，我们检测了长日光暗循环和温度循环下维管系统和整个叶片中昼夜期 CAB 表达计时 1 (TOC1) 的表达。我们发现脉管系统时钟在明暗循环下有提前的 TOC1 相位，但在温度循环下则没有，这表明脉管系统时钟对温度信号的敏感性较低。此外，TOC1的相位提前仅在长日照条件下出现，而在短日照条件下则没有。这些结果支持我们之前的结论，即脉管系统中的生物钟优先感知光周期信号。
Tissue-specific functions of the circadian clock in Arabidopsis have recently been revealed. The vasculature clock shows distinctive gene expression profiles compared to the clock in other tissues under light-dark cycles. However, it has not yet been established whether the vasculature clock also shows unique gene expression patterns that correlate with temperature cycles, another important environmental cue. Here, we detected diel phase of TIMING OF CAB EXPRESSION 1 (TOC1) expression in the vasculature and whole leaf under long-day light-dark cycles and temperature cycles. We found that the vasculature clock had advanced TOC1 phase under light-dark cycles but not under temperature cycles, suggesting that the vasculature clock has lower sensitivity against temperature signals. Furthermore, the phase advancement of TOC1 was seen only under long-day condition but not under short-day condition. These results support our previous conclusion that the circadian clock in vasculature preferentially senses photoperiodic signals.