TGF-β1 enhances cardiomyogenic differentiation of skeletal muscle-derived adult primitive cells
TGF-β1 enhances cardiomyogenic differentiation of skeletal muscle-derived adult primitive cells
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DOI：
10.1007/s00395-008-0729-9
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发表时间：
2008-11-01
影响因子：
9.5
通讯作者：
Dawn, Buddhadeb
中科院分区：
文献类型：
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作者：
Abdel-Latif, Ahmed;Zuba-Surma, Ewa K.;Dawn, Buddhadeb
成体原始细胞向心脏分化的最佳条件尚待确定。我们定量比较了胰岛素样生长因子-1、强啡肽B、胰岛素、催产素、碱性成纤维细胞生长因子和转化生长因子-β1诱导心肌细胞分化的效果。成年小鼠骨骼肌源性Sca1+/CD45-/c-kit-/Thy-1+(SM-)和Sca1-/CD45-/c-kit-/Thy-1+(SM-)细胞分别在基础培养液(BM；DMEM、FBS、IGF-1、强啡肽B)和BM+胰岛素、催产素、碱性成纤维细胞生长因子或转化生长因子-β1的基础上培养。BM+转化生长因子-β1可上调NKX2.5、GATA-4和Myl2的mRNA表达。30天后，BM+转化生长因子-β1诱导SM-细胞分化的程度最大(通过心脏标志物的形态和表达)。我们得出结论，转化生长因子-β1促进了骨骼肌源性成人原始细胞的心肌分化。这一策略可用于诱导心脏分化，以及检测成人组织来源的干细胞/祖细胞的心肌分化潜能。
The optimal medium for cardiac differentiation of adult primitive cells remains to be established. We quantitatively compared the efficacy of IGF-1, dynorphin B, insulin, oxytocin, bFGF, and TGF-beta 1 in inducing cardiomyogenic differentiation. Adult mouse skeletal muscle-derived Sca1+/CD45-/c-kit-/Thy-1+ (SM+) and Sca1-/CD45-/c-kit-/Thy-1+ (SM-) cells were cultured in basic medium (BM; DMEM, FBS, IGF-1, dynorphin B) alone and BM supplemented with insulin, oxytocin, bFGF, or TGF-beta 1. Cardiac differentiation was evaluated by the expression of cardiac-specific markers at the mRNA (qRT-PCR) and protein (immunocytochemistry) levels. BM+TGF-beta 1 upregulated mRNA expression of Nkx2.5 and GATA-4 after 4 days and Myl2 after 9 days. After 30 days, BM+TGF-beta 1 induced the greatest extent of cardiac differentiation (by morphology and expression of cardiac markers) in SM- cells. We conclude that TGF-beta 1 enhances cardiomyogenic differentiation in skeletal muscle-derived adult primitive cells. This strategy may be utilized to induce cardiac differentiation as well as to examine the cardiomyogenic potential of adult tissue-derived stem/progenitor cells.