FT-IR spectroscopic characteristics of differently cultivated Bacillus subtilis
FT-IR spectroscopic characteristics of differently cultivated Bacillus subtilis
复制标题
DOI：
10.1016/j.micres.2004.05.002
复制
发表时间：
2004-01-01
影响因子：
6.7
通讯作者：
Kubat, J
中科院分区：
文献类型：
--
作者：
Filip, Z;Herrmann, S;Kubat, J
枯草芽孢杆菌是一种广泛分布在不同环境中的需氧内生孢子形成细菌。由于它代表了具有一定健康相关性的生物制剂，因此非常需要对其进行快速检测和识别。为此目的，通过使用 FT-IR 光谱法，从在不同组成的培养基中生长并在细菌的不同阶段收获的细胞团获得了略有不同的特征。生长。如果均匀培养，即在最低浓度的营养肉汤中于30℃培养24小时，细胞。大量枯草芽孢杆菌输送到井中。差异光谱，其中核酸结构的主要吸收带位于3300cm(-1)，细胞壁成分的主要吸收带位于3000-2800cm(-1)，蛋白质结构的主要吸收带位于1660、1544和1235cm(-1)，以及一些脂肪族结构单元的吸收带位于1080cm(-1)。衰减总扫描、反射扫描和吸收/透射扫描技术提供了结构相同的光谱，但通过前一种技术获得的光谱表达更清晰。 (C) 2004 年爱思唯尔有限公司。版权所有。
Bacillus subtilis is an aerobic endospore forming bacterium widely spread in different environments. Because it represents a biological agent of some health relevance, its rapid detection and identification is highly desirable. By using FT-IR spectroscopy for this purpose slightly different characteristics were obtained from cell mass grown in differently composed cultural media, and harvested in different phases of bacterial. growth. If cultivated uniformly, i.e., 24h at 30degreesC in a minimum-strength nutrient broth, cell. mass of B. subtilis delivered a well. differentiated spectrum with major absorption bands of nucleic acid structures at 3300cm(-1), cell wall constituents at 3000-2800cm(-1), proteinaceous structures at 1660, 1544 and 1235cm(-1), and some aliphatic structural units at 1080cm(-1). Attenuated total, reflectance, and absorption/ transmission scanning techniques, delivered structurally identical spectra but those obtained by the former technique were more expressed. (C) 2004 Elsevier GmbH. All rights reserved.