Pulsatile secretion of thyrotropin during fasting: a decrease of thyrotropin pulse amplitude.
Pulsatile secretion of thyrotropin during fasting: a decrease of thyrotropin pulse amplitude.
复制标题
禁食期间促甲状腺素的脉动分泌：促甲状腺素脉冲幅度降低。
DOI：
10.1210/jcem-70-6-1631
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
W. Wiersinga
中科院分区：
文献类型：
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作者：
J. Romijn;R. Adriaanse;G. Brabant;K. Prank;Erik Endert;W. Wiersinga
在八名健康受试者（卵泡期的四名男性和四名女性）中研究了禁食对昼夜节律和脉动 TSH 分泌的影响。每个受试者都接受了两次研究，一次是在 24 小时内正常进食，另一次是在 60 小时禁食的最后 24 小时内。 24 小时内每 10 分钟采集一次血液样本，通过灵敏的免疫放射测定法测量 TSH。禁食导致血浆 T3 降低 [1.73 +/- 0.06 vs. 1.36 +/- 0.04 nmol/L； P 小于 0.01（平均值 +/- SE），对照期与禁食]和甲状腺球蛋白（52 +/- 8 与 35 +/- 7 pmol/L；P 小于 0.001），血浆 rT3 增加（0.30 +/- 0.06 与 0.44 +/- 0.09 nmol/L；P 小于 0.02）。两个时期的血浆T4、甲状腺激素结合指数和游离T4没有统计学差异。禁食期间平均血浆 24 小时 TSH 浓度低于对照期（2.0 +/- 0.3 与 1.0 +/- 0.2 mU/L；P 小于 0.005）。这与禁食期间平均 TSH 脉搏幅度的降低相关（Desade 方案：0.6 +/- 0.1 对比 0.3 +/- 0.1 mU/L；P 小于 0.01；聚类方案：0.5 +/- 0.1 对比 0.2 +/- 0.1 mU/L；P 小于 0.05），而禁食期间 TSH 脉搏频率没有变化（Desade 方案：8.4） +/- 0.9 与 9.8 +/- 0.8 脉冲/24 小时；聚类程序：9.5 +/- 0.5 与 7.9 +/- 0.9 脉冲/24 小时）。在对照期和禁食期间，平均 24 小时 TSH 浓度与平均 TSH 脉冲幅度之间存在高度显着的相关性。尽管禁食期间 TSH 浓度在 24 小时内明显下降，但禁食尤其降低了夜间 TSH 激增的绝对值（1.3 +/- 0.3 对比 0.4 +/- 0.1 mU/L，P 小于 0.02）和相对值（101 +/- 18% 对比 40 +/- 14%；P 小于 0.02）（平均 TSH 0000-0400 h）与平均 TSH 1500-1900 h) 相比。禁食期间夜间 TSH 峰值的降低与 TSH 脉冲幅度的显着降低相关，但 TSH 脉冲数量在 2000-0400 小时之间没有变化。总之，在血浆 T4 浓度没有变化的情况下，禁食会减少 24 小时 TSH 分泌和夜间 TSH 激增。这与 TSH 脉冲幅度降低有关，而 TSH 脉冲频率保持不变。
The effect of fasting on circadian and pulsatile TSH secretion was investigated in eight healthy subjects (four men and four women in the follicular phase). Each subject was studied twice, once during 24 h with normal food intake and once during the last 24 h of a 60-h fast. Blood was sampled every 10 min during 24 h for measurement of TSH by a sensitive immunoradiometric assay. Fasting induced a decrease in plasma T3 [1.73 +/- 0.06 vs. 1.36 +/- 0.04 nmol/L; P less than 0.01 (mean +/- SE), control period vs. fasting] and thyroglobulin (52 +/- 8 vs. 35 +/- 7 pmol/L; P less than 0.001) and an increase in plasma rT3 (0.30 +/- 0.06 vs. 0.44 +/- 0.09 nmol/L; P less than 0.02). Plasma T4, thyroid hormone binding index, and free T4 were not statistically different in both periods. The mean plasma 24-h TSH concentration was lower during fasting than in the control period (2.0 +/- 0.3 vs. 1.0 +/- 0.2 mU/L; P less than 0.005). This was associated with a decrease in mean TSH pulse amplitude during fasting (Desade program: 0.6 +/- 0.1 vs. 0.3 +/- 0.1 mU/L; P less than 0.01; Cluster program: 0.5 +/- 0.1 vs. 0.2 +/- 0.1 mU/L; P less than 0.05), whereas TSH pulse frequency during fasting was unchanged (Desade program: 8.4 +/- 0.9 vs. 9.8 +/- 0.8 pulses/24 h; Cluster program: 9.5 +/- 0.5 vs. 7.9 +/- 0.9 pulses/24 h). There was a highly significant correlation between the mean 24-h TSH concentration and the mean TSH pulse amplitude during both the control period and fasting. Although the decrease in TSH concentration during fasting was evident over 24 h, fasting especially decreased the absolute (1.3 +/- 0.3 vs. 0.4 +/- 0.1 mU/L, P less than 0.02) and the relative (101 +/- 18% vs. 40 +/- 14%; P less than 0.02) nocturnal TSH surge (mean TSH 0000-0400 h vs. mean TSH 1500-1900 h). The decreased nocturnal TSH surge during fasting was associated with a significantly decreased TSH pulse amplitude, but with an unaltered number of TSH pulses between 2000-0400 h. In conclusion, fasting decreases 24-h TSH secretion and the nocturnal TSH surge in the absence of a change in plasma T4 concentration. This is associated with a decreased TSH pulse amplitude, whereas TSH pulse frequency remains unchanged.