Quantitative 3D imaging of yeast by hard X-ray tomography
Quantitative 3D imaging of yeast by hard X-ray tomography
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DOI：
10.1002/jemt.21108
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发表时间：
2012-05-01
影响因子：
2.5
通讯作者：
Tian, Yangchao
中科院分区：
文献类型：
--
作者：
Zheng, Ting;Li, Wenjie;Tian, Yangchao
全视场硬X射线层析成像可用于获得生物样品的三维（3D）纳米级结构。基于Zernike相位衬度成像技术和重金属染色方法，在8 keV能量下获得了优于50 nm的空间分辨率，清晰地显示了裂殖酵母裂殖酵母的图像。细胞器在细胞周期中的分布和形状清晰可见，并区分两种类型的细胞器。比较不同时期细胞的结果，并定量分析细胞器体积与细胞体积的比值。结果表明，随着两种细胞器的形状和大小的变化，在生长期和分裂期，细胞器的比例保持不变，而在稳定期，细胞器的比例急剧增加。我们的研究结果表明，硬X射线显微镜成像和揭示生物样品的结构信息是一种补充方法。Microsc. Res. Tech.，2012. (c)2011 Wiley Periodicals，Inc.
Full-field hard X-ray tomography could be used to obtain three-dimensional (3D) nanoscale structures of biological samples. The image of the fission yeast, Schizosaccharomyces pombe, was clearly visualized based on Zernike phase contrast imaging technique and heavy metal staining method at a spatial resolution better than 50 nm at the energy of 8 keV. The distributions and shapes of the organelles during the cell cycle were clearly visualized and two types of organelle were distinguished. The results for cells during various phases were compared and the ratios of organelle volume to cell volume can be analyzed quantitatively. It showed that the ratios remained constant between growth and division phase and increased strongly in stationary phase, following the shape and size of two types of organelles changes. Our results demonstrated that hard X-ray microscopy was a complementary method for imaging and revealing structural information for biological samples. Microsc. Res. Tech., 2012. (c) 2011 Wiley Periodicals, Inc.