Satellite RNA-mediated resistance to turnip crinkle virus in Arabidopsis involves a reduction in virus movement.
Satellite RNA-mediated resistance to turnip crinkle virus in Arabidopsis involves a reduction in virus movement.
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DOI：
10.1105/tpc.9.11.2051
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发表时间：
1997-11
期刊：
影响因子：
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通讯作者：
Q. Kong;Jianlong Wang;Anne E. Simon
中科院分区：
文献类型：
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作者：
Q. Kong;Jianlong Wang;Anne E. Simon
卫星RNA（sat-RNA）是病毒的寄生虫，可以介导对辅助病毒的抗性。我们以前表明，一个卫星RNA（卫星RNA C）的芜菁皱纹病毒（TCV），这通常会加剧症状的TCV，是能够减轻症状时，TCV包含的外壳蛋白（CP）的碎米荠褪绿斑点病毒（TCV-CPCCFV）。我们现在已经确定，sat-RNA C也减轻TCV的症状，包括CP开放阅读框架（TCV-CPm）的起始AUG的改变。TCV-CPm能够在TCV敏感生态型哥伦比亚（Col-0）和TCV抗性生态型第戎（Di-0）中系统移动，在受感染的植物中产生降低水平的CP并且没有检测到病毒粒子。Sat-RNA C使原生质体中TCV-CPm的积累降低< 25%，而使Col-0和Di-0的未接种叶片中TCV-CPm的水平降低90至100%。我们的研究结果表明，在一个可能改变CP的水平降低的存在下，sat-RNA C减少病毒的长距离移动的方式是独立的水杨酸依赖的防御途径。
Satellite RNAs (sat-RNAs) are parasites of viruses that can mediate resistance to the helper virus. We previously showed that a sat-RNA (sat-RNA C) of turnip crinkle virus (TCV), which normally intensifies symptoms of TCV, is able to attenuate symptoms when TCV contains the coat protein (CP) of cardamine chlorotic fleck virus (TCV-CPCCFV). We have now determined that sat-RNA C also attenuates symptoms of TCV containing an alteration in the initiating AUG of the CP open reading frame (TCV-CPm). TCV-CPm, which is able to move systemically in both the TCV-susceptible ecotype Columbia (Col-0) and the TCV-resistant ecotype Dijon (Di-0), produced a reduced level of CP and no detectable virions in infected plants. Sat-RNA C reduced the accumulation of TCV-CPm by < 25% in protoplasts while reducing the level of TCV-CPm by 90 to 100% in uninoculated leaves of Col-0 and Di-0. Our results suggest that in the presence of a reduced level of a possibly altered CP, sat-RNA C reduces virus long-distance movement in a manner that is independent of the salicylic acid-dependent defense pathway.