Bile alcohol metabolism in man. Conversion of 5beta-cholestane-3alpha, 7alpha,12alpha, 25-tetrol to cholic acid.
Bile alcohol metabolism in man. Conversion of 5beta-cholestane-3alpha, 7alpha,12alpha, 25-tetrol to cholic acid.
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人的胆汁醇代谢。
DOI：
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发表时间：
1975
影响因子：
15.9
通讯作者：
E. Mosbach
中科院分区：
文献类型：
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作者：
G. Salen;S. Shefer;T. Setoguchi;E. Mosbach
为了研究C25-羟基胆汁醇作为胆酸前体的作用，对两名脑腱黄瘤病患者和两名正常人静脉注射[G-3-H]5beta-cholestane-3alpha，7alpha12alpha，25-tetrol。脉冲标记后1天，从CTX受试者的胆汁和粪便中提取的胆酸和正常人的胆汁中均有放射性。在两名正常人中，[G-3-H]-胆酸的SPACT衰变曲线呈指数型，未检测到[G-3-H]-5beta-cholestane-3alpha，7alpha，12alpha，25-tetrol。相比之下，CTX受试者的胆汁和粪便中有相当数量的标记5β-胆甾烷-3α，-7α，12α，25-四氢呋喃。粪便[G-3-H]5beta-cholestane-3alpha，7alpha，12alpha，25-tetrol和[G-3-H]-胆酸的SPACT-时间曲线符合前体-产物关系。虽然这些结果提示5beta-cholestane-3alpha，7alpha，12alpha，25-tetrol可能是人类胆酸的前体，但不能排除C26-羟基中间体代表正常途径的可能性。
To study the role of C25-HYDROXY BILE ALCOHOLS AS PRECURSORS OF CHOlic acid, [G-3-H]5beta-cholestane-3alpha,7alpha12alpha,25-tetrol was administered intravenously to two subjects with cerebrotendinous xanthomatosis (CTX) and two normal individuals. One day after pulse labeling, radioactivity was present in the cholic acid isolated from the bile and feces of the subjects with CTX and the bile of the normal individuals. In the two normal subjects, the sp act decay curves of [G-3-H]-cholic acid were exponential, and no traces of [G-3-H]-5beta-cholestane-3alpha,7alpha,12alpha,25-tetrol were detected. In contrast, appreciable quantities of labeled 5beta-cholestane-3alpha,-7aopha,12alpha,25-tetrol were present in the bile and feces of the CTX subjects. The sp act vs. time curves of fecal [G-3-H]5beta-cholestane-3alpha,7alpha,12alpha,25-tetrol and [G-3-H]-cholic acid showed a precursor-product relationship. Although these results suggest that 5beta-cholestane-3alpha,7alpha,12alpha,25-tetrol may be a precursor of cholic acid in man, the possibility that C26-hydroxy intermediates represent the normal pathway can not be excluded.